Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of compatibility of Clostridium butyricum and Bacillus subtilis on growth performance, lipid metabolism, antioxidant status and cecal microflora of broilers during the starter phase

  • Xu Zhao (College of Agriculture and Forestry Science, Linyi University) ;
  • Jiarong Zhuang (Linyi Backbone Biotechnology Co., Ltd.) ;
  • Faling Zhang (Shandong Lonct Enzymes Co., Ltd.) ;
  • Hongtao Li (Linyi Backbone Biotechnology Co., Ltd.) ;
  • Juan Yu (Linyi Backbone Biotechnology Co., Ltd.) ;
  • Chengli Wang (College of Agriculture and Forestry Science, Linyi University) ;
  • Tengjiao Lv (College of Agriculture and Forestry Science, Linyi University) ;
  • Qingzhen Li (College of Agriculture and Forestry Science, Linyi University) ;
  • Jimei Zhang (College of Agriculture and Forestry Science, Linyi University)
  • Received : 2024.02.29
  • Accepted : 2024.06.20
  • Published : 2024.11.01
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Abstract

Objective: This study aimed to determine the effects of compatibility of Clostridium butyricum and Bacillus subtilis on growth performance, lipid metabolism, antioxidant status and cecal microflora of broilers during the starter phase. Methods: A total of 600 1-day-old Ross 308 broilers were randomly divided into two groups with six replicates in each group. Chickens in the control group were fed a basal diet, while chickens in the experimental group were fed a diet supplemented with 2×108 colony forming units (CFU)/kg of C. butyricum and 1×109 CFU/kg of B. subtilis. The experimental period was 21 days. Results: Addition of C. butyricum and B. subtilis significantly increased (p<0.05) the body weight and liver nicotinamide adenine dinucleotide phosphate-malic enzyme (NADP-ME) activity of broilers, enhanced (p<0.05) the average daily gain and average daily feed intake of broilers. However, the addition of C. butyricum and B. subtilis did not significantly affect the concentrations of triglyceride and total cholesterol in the serum, the activities of fatty acid synthase and acetyl-CoA carboxylase in the liver, the total antioxidant capacity, glutathione peroxidase activity and malondialdehyde content in the serum and liver. Besides, microbial analysis revealed that supplementation of C. butyricum and B. subtilis increased (p<0.05) the abundance of Firmicutes such as CHKCI001 and Faecalibacterium, decreased (p<0.05) the abundance of Bacteroidota such as Bacteroides and Alistipes. Spearman correlation analysis confirmed that the above cecal microbiota were closely related to the growth performance of broilers (p<0.05). In addition, simultaneous supplementation of C. butyricum and B. subtilis significant affected (p<0.05) 33 different functional pathways such as lipid metabolism and carbohydrate metabolism. This explains the phenomenon of increased growth performance and liver NADP-ME activity in the probiotics group. Conclusion: The compatibility of C. butyricum and B. subtilis could improve the growth of broilers during the starter phase by changing the cecal microflora.

Keywords

Acknowledgement

This work was supported by the Natural Science Foundation of Shandong Province (grant number ZR2021MC170); and the Scientific Research Start-up Project of High-level Talents (High-level Doctor) of Linyi University (grant number LYDX 2019BS036).

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