The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Exploration of the Potential and Mechanisms of Diabetic Cognitive Disorder Modulation by Daehwangmokdanpi-tang through a Network Pharmacological Approach

네트워크 약리학적 접근을 통한 대황목단피탕(大黃牧丹皮湯)의 당뇨병성 인지장애 조절 가능성 및 기전 탐색

  • Yebin Lim (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Bitna Kweon (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Dong-Uk Kim (Department of Pharmacology, College of Korean Medicine, Wonkwang University) ;
  • Do-Eun Lee (Department of Korean Neuropsychiatry Medicine, College of Korean Medicine, Wonkwang University) ;
  • Jungtae Leem (Research center of Traditional Korean medicine, Wonkwang University) ;
  • Dong-Gu Kim (Department of Herbology, College of Korean Medicine, Dong-Eui University) ;
  • Hyung Won Kang (Department of Korean Neuropsychiatry Medicine, College of Korean Medicine, Wonkwang University) ;
  • Gi-Sang Bae (Department of Pharmacology, College of Korean Medicine, Wonkwang University)
  • 임예빈 (원광대학교 한의과대학 약리학교실) ;
  • 권빛나 (원광대학교 한의과대학 약리학교실) ;
  • 김동욱 (원광대학교 한의과대학 약리학교실) ;
  • 이도은 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 임정태 (원광대학교 한국전통의학연구소) ;
  • 김동구 (동의대학교 한의과대학 본초학교실) ;
  • 강형원 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 배기상 (원광대학교 한의과대학 약리학교실)
  • Received : 2024.04.15
  • Accepted : 2024.05.07
  • Published : 2024.06.01
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Abstract

Objectives: This study utilized a network pharmacology approach to investigate the potential therapeutic effects and underlying mechanisms of Daehwangmokdanpi-tang (DHMDPT) in diabetic cognitive disorder (DCD). Methods: The compounds of DHMDPT and their target genes were obtained from the OASIS and PubChem databases. These putative target genes were compared with known targets of DCD to identify potential correlations. Using Cytoscape 3.10.2, a network was constructed to highlight key target genes. To further elucidate the underlying mechanisms, functional enrichment analysis was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, CB-DOCK was used to assess binding affinities and confirm the interactions. Results: The results showed that a total of 27 compounds and 439 related genes were identified from DHMDPT. Among these, 373 genes interacted with the DCD gene set, indicating a close relationship between the effects of DHMDPT and DCD. Through GO enrichment analysis and KEGG pathways, 'Regulation of Apoptotic Process', 'Cytokine-Mediated signaling pathway', and 'AGE-RAGE signaling pathway in diabetic complications' were identified as the functional pathways of the 18 key target genes of DHMDPT on DCD. Additionally, molecular docking was performed to assess the binding affinities of the six most highly associated key target genes of DCD with active compounds. Conclusions: Using a network pharmacology approach, which included molecular docking, DHMDPT was found to be highly relevant to DCD. This study could serve as a foundation for further research on the cognitive enhancement effects of DHMDPT in DCD.

Keywords

Acknowledgement

본 연구는 보건복지부의 재원으로 한국보건산업진흥원의 보건의료기술(과제고유번호: HF20C0212)과 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(NRF-2021R1I1A2053285/RS-2023-00248483).

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