한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제37권5호
  • /
  • Pages.255-265
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  • 2024
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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흑후추에서 분리한 글루칸형 다당류가 대식세포 자극 활성에 미치는 영향

Effects of Glucan-type Polysaccharides from Black Pepper (Piper nigrum) on Macrophage Stimulating Activity

  • 김다은 (국립한국교통대학교 식품영양학전공) ;
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) ;
  • 정원비 (국립한국교통대학교 식품영양학전공) ;
  • 김효원 (국립한국교통대학교 식품영양학전공) ;
  • 문수민 (국립한국교통대학교 식품영양학전공) ;
  • 신광순 (경기대학교 식품생물공학과) ;
  • 유광원 (국립한국교통대학교 식품영양학전공)
  • Da Eun Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyun Young Shin (Transdisciplinary Major in Learning Health Systems, Dept. of Integrated Biomedical and Life Science, Graduate School, Korea University) ;
  • Won Bi Jeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyo Won Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Su Min Moon (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Kwang-Soon Shin (Dept. of Food Science and Biotechnology, Kyonggi University) ;
  • Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation)
  • 투고 : 2024.09.14
  • 심사 : 2024.10.10
  • 발행 : 2024.10.31
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초록

To utilize pepper (Piper nigrum) as an immunostimulatory agent, we isolated macrophage stimulating polysaccharides from pepper and investigated their macrophage activating activities. Hot-water extracts (HW) of black pepper (BP) and white pepper (WP) were prepared, and their macrophage stimulating activities were evaluated using RAW 264.7 cells. BP-HW significantly promoted the secretion of macrophage stimulating factors such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-12 compared to WP-HW. When BP and WP-HW were fractionated into crude polysaccharides (CP) and low molecules (LM) by ethanol precipitation, BP-CP demonstrated significantly more potent activity. Furthermore, BP-CP not only induced mRNA gene expression of macrophage activation factors, but also promoted nuclear localization of p65 and c-Jun. In addition, component sugar analysis revealed that glucan-type polysaccharides in BP-CP played a crucial role in macrophage activation. Taken together, these findings suggest that black pepper has industrial applicability not only as a spice, but also as an immunostimulatory functional material.

키워드

과제정보

본 논문은 2024년 국립한국교통대학교 지원을 받아 수행하였음.

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