DOI QR코드

DOI QR Code

Antioxidant Compounds and Antioxidant Activities of Rapeseed Plants according to Cultivar and Growth Stage

유채 품종 및 생육단계별 식물체의 항산화 성분 및 항산화 특성

  • Gyeong-dan Yu (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Eom-ji Hwang (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • You-jin Park (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Jae Hee Jeong (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Da Hee An (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Hyun-Joo Kim (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Yeon-Sang Song (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Koan Sik Woo (Planning and Coordination Div., National Institute of Crop Science, RDA)
  • 유경단 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 황엄지 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 박유진 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 정재희 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 안다희 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 김현주 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 송연상 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 우관식 (농촌진흥청 국립식량과학원 기획조정과)
  • Received : 2024.09.03
  • Accepted : 2024.10.07
  • Published : 2024.10.31

Abstract

This study investigated phenolic compounds and radical scavenging activities of rapeseed plants according to cultivar and growth stage. For 1st, 2nd, and 3rd harvested rapeseed plants, total polyphenol contents were 12.56~15.85, 10.28~13.17, and 9.94~16.01 mg gallic acid equivalents/g sample, respectively; total flavonoid contents were 9.86~11.05, 10.41~13.06, and 8.18~16.64 mg catechin equivalents/g sample, respectively; total chlorophyll contents were 17.26~21.66, 19.31~24.95, and 22.16~38.39 mg/g sample, respectively; and total glucosinolates contents were 31.13~46.22, 28.51~34.72, and 22.18~30.58 mg SE/g sample, respectively. DPPH and ABTS radical scavenging activities of rapeseed plants were 3.26~5.91 and 10.69~17.17 mg trolox equivalents/g sample, respectively. FRAP activities of 11st, 2nd, and 3rd harvested rapeseed plants were 142.88~63.93, 37.97~61.24, and 32.93~52.36 mM/g sample, respectively. Phenolic compounds and radical scavenging activities of rapeseed plants were found to be high in Halla, Yuryeo, and Yeongsan cultivars. Overall, phenolic compounds and radical scavenging activities of rapeseed plants tended to decrease as the harvest time was delayed. These results indicate that more research on functional ingredients and physiological activities of rapeseed plants is needed to diversify their fields of use.

Keywords

Acknowledgement

본 논문은 농촌진흥청 시험연구사업(과제번호: PJ017422042024)의 지원에 의해 이루어진 것임.

References

  1. An DH, Yu GD, Kim KS, Cha YL, Jeong JH, Choi JB, Woo KS, Hwang EJ, Park YJ. 2023. Quality characteristics of rapeseed oils according to different roasting temperatures. Korean J Food Nutr 36:479-488
  2. Bhandari SR, Kwak JH. 2015. Chemical composition and antioxidant activity in different tissues of Brassica vegetables. Molecules 20:1228-1243
  3. Bystrom LM, Guzman ML, Rivella S. 2014. Iron and reactive Oxygen species: Friends or foes of cancer cells? Antioxid Redox Signaling 20:1917-1924
  4. Chung IM, Rekha K, Rajakumar G, Thiruvengadam M. 2016. Production of glucosinolates, phenolic compounds and associated gene expression profiles of hairy root cultures in turnip (Brassica rapa ssp. rapa). 3 Biotech 6:175
  5. Clarke JD, Dashwood RH, Ho E. 2008. Multi-targeted prevention of cancer by sulforaphane. Cancer Lett 269:291-304
  6. Duval B, Shetty K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed andise root extract. J Food Biochem 25:361-377
  7. Falcinelli B, Sileoni V, Marconi O, Perretti G, Quinet M, Lutts S, Benincasa P. 2017. Germination under moderate salinity increases phenolic content and antioxidant activity in rapeseed (Brassica napus var. oleifera Del.) sprouts. Molecules 22:1377
  8. Fenwick GR, Heaney RK, Mullin WJ. 1983. Glucosinolates and their breakdown products in food and food plants. Crit Rev Food Sci Nutr 18:123-201
  9. Ferreres F, Valentao P, Llorach R, Pinheiro C, Cardoso L, Pereira JA, Sousa C, Seabra RM, Andrade PB. 2005. Phenolic compounds in external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC). J Agric Food Chem 53:2901-2907
  10. Francisco M, Moreno DA, Cartea ME, Ferreres F, Garcia-Viguera C, Velasco P. 2009. Simultaneous identification of glucosinolates and phenolic compounds in a representative collection of vegetable Brassica rapa. J Chromatogr A 1216:6611-6619
  11. Han N, Woo KS, Lee JY, Song SB, Lee YY, Kim M, Kang MS, Kim HJ. 2022. Comparison of physicochemical characteristics, functional compounds, and physiological activities in adzuki bean cultivars. J Korean Soc Food Sci Nutr 51:428-438
  12. Harbaum B, Hubbermann EM, Zhu Z, Schwarz K. 2008. Free and bound phenolic compounds in leaves of pak choi (Brassica campestris L. ssp. chinensis var. communis) and Chinese leaf mustard (Brassica juncea Coss). Food Chem 110:838-846
  13. Hayes JD, Kelleher MO, Eggleston IM. 2008. The cancer chemopreventive actions of phytochemicals derived from glucosinolates. Eur J Nutr 47:73-88
  14. Hwang EJ, Kim S, Kim HJ, Jeong J, Lee YH, Ha TJ, Woo KS. 2022. Quality and antioxidant characteristics according to different harvest periods and steaming treatment of apios (Apios americana Medikus) cultivated in Korea. Korean J Food Nutr 35:445-452
  15. Hwang EJ, Kim TH, Park W, Lee KH, Nam SS, Park YJ, Kim S, Lee HU, Chung MN, Ha TJ, Woo KS. 2023. Antioxidant characteristics of sweet potato (Ipomoea batatas (L.) Lam.) according to different plant parts and drying methods. Korean J Food Nutr 36:327-333
  16. Hwang ES. 2010. Changes in myrosinase activity and total glucosinolate levels in Korean Chinese cabbages by salting conditions. Korean J Food Cookery Sci 26:104-109
  17. Hwang ES, Lee HJ. 2006. Phenylethyl isothiocyanate and its N-acetylcysteine conjugate suppress the metastasis of SK-Hep1 human hepatoma cells. J Nutr Biochem 17:837-846
  18. Jeong J, An DH, Cha YL, Choi JB, Kim SY, Kim KS. 2024. 'Yuryeo': A rapeseed with high oleic acid, resistant to Sclerotinia stem rot and lodging. Korean J Breed Sci 56:53-61
  19. Kim HJ, Lee JH, Lee BW, Lee YY, Jeon YH, Lee BK, Woo KS. 2018. Quality and physicochemical characteristics of the Korean cowpea cultivars grown in different seeding periods. Korean J Food Nutr 31:502-510
  20. Kim JE, Joo SI, Seo JH, Lee SP. 2009. Antioxidant and α- glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr 38:989-995
  21. Kim KS, Jang YS, Lee YH, Kim CW, Choi KH, Kang DS, Kim ST, Choi IH. 2014. A rapeseed intermediate parent 'Jungmo 7001' with wide adaptable and large flower. Korean J Breed Sci 46:302-306
  22. Kim KS, Jang YS, Lee YH, Seo TC, Choi KH, Kang DS, Kim ST, Lee KB. 2015. A rapeseed intermediate parent 'Jungmo 7002' with flowering uniformity and lodging tolerance. Korean J Breed Sci 47:276-280
  23. Lee JY, Woo KS, Seo JH, Lee YY, Lee BW, Kim MH, Kang MS, Kim HJ. 2021. Physicochemical qualities and physiological activities of black soybeans by cultivation area and cultivars. J Korean Soc Food Sci Nutr 50:29-35
  24. Llorach R, Espin JC, Tomas-Barberan FA, Ferreres F. 2003. Valorization of cauliflower (Brassica oleracea L. var. botrytis) by-products as a source of antioxidant phenolics. J Agric Food Chem 51:2181-2187
  25. Middleton E. 1994. Potential health-promoting properties of citrus flavonoids. Food Technol 48:115-119
  26. Moon GS, Kwon TW, Ryu SH. 2003. Comparison of antioxidative activities of soybean components by different assays. Korean Soybean Dig 20:28-36
  27. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-savenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71:109-114
  28. Park BH, Koh KM, Jeon ER. 2018. Quality characteristics of wet noodles added with sword bean powder. J Korean Soc Food Cult 33:374-381
  29. Park S, Arasu MV, Jiang N, Choi SH, Lim YP, Park JT, Al-Dhabi NA, Kim SJ. 2014. Metabolite profiling of phenolics, anthocyanins and flavonols in cabbage (Brassica oleracea var. capitata). Ind Crops Prod 60:8-14
  30. Park YJ, Hwang EJ, Yu GD, Woo KS. 2024. Quality characteristics and antioxidant characteristics of sword bean tea distributed in domestic markets. Korean J Food Nutr 37:100-109
  31. Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152-159
  32. Roh KH, Kwak BK, Kim HU, Lee KR, Kim SH, Suh MC, Kim H, Kim JB. 2011. Production of transgenic plants in Brassica napus winter cultivar 'Youngsan'. J Appl Biol Chem 54:26-32
  33. Rural Development Administration. 2008. Standard Farming Textbook-167. Rapeseed Cultivation. pp.52-69. Rural Development Administration
  34. Samanta A, Das G, Das SK. 2011. Roles of flavonoids in plants. Int J Pharm Sci Tech 6:12-35
  35. Shim KH, Sung NK, Kang KS, Ahn CW, Seo KI. 1992. Analysis of glucosinolates and the change of contents during processing and storage in Cruciferous vegetables. J Korean Soc Food Nutr 21:43-48
  36. Sola I, Vujcic Bok V, Dujmovic M, Rusak G. 2020. Developmentally-related changes in phenolic and L-ascorbic acid content and antioxidant capacity of Chinese cabbage sprouts. J Food Sci Technol 57:702-712
  37. Sousa C, Pereira DM, Pereira JA, Bento A, Rodrigues MA, Dopico-Garcia S, Valentao P, Lopes G, Ferreres F, Seabra RM, Andrade PB. 2008. Multivariate analysis of tronchuda cabbage (Brassica oleracea L. var. costata DC) phenolics: Influence of fertilizers. J Agric Food Chem 56:2231-2239
  38. Stoewsand GS. 1995. Bioactive organosulfur phytochemicals in Brassica oleracea vegetables: A review. Food Chem Toxicol 33:537-543
  39. Vallejo F, Tomas-Barberan FA, Garcia-Viguera C. 2003. Effect of climatic and sulfur fertilisation conditions, on phenolic compounds and vitamin C, in the inflorescences of eight broccoli cultivars. Eur Food Res Technol 216:395-401
  40. Woo KS, Bae HH, Jung GH, Son BY, Kim HJ. 2021. Quality and physicochemical characteristics of Korean maize hybrids according to the seed and pollen parent. Korean J Food Nutr 34:407-414
  41. Woo KS, Ko JY, Kim HY, Lee YH, Jeong HS. 2013. Changes in quality characteristics and chemical components of sweet potatoes cultivated using different methods. Korean J Food Sci Technol 45:305-311
  42. Woo KS, Song SB, Oh BG, Seo MC, Ko JY, Lee JS, Kang JR, Nam MH, Jeong HS. 2009. Antioxidant activity of ethanol extraction from horseweed (Erigeron canadensis L.) with pretreatment conditions. J Korean Soc Food Sci Nutr 38:1279-1283
  43. Zhang M, Zheng C, Yang M, Zhou Q, Li W, Liu C, Huang F. 2019. Primary metabolites and polyphenols in rapeseed (Brassica napus L.) cultivars in China. J Am Oil Chem Soc 96:303-317