Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Differential Asthmatic Effect Due to House Dust Mite Depending on Age and Exposure Duration

  • Ji-Sook Lee (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • Received : 2024.08.05
  • Accepted : 2024.09.25
  • Published : 2024.09.30
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Abstract

Asthma is a chronic lung disease characterized by wheezing, cough, and chest pain. The objective of this study was to examine asthmatic effect due to house dust mite (HDM) according to age and exposure duration. Asthma induction in young (6 weeks) and old (12 months) aged C57BL6/J mice was accomplished by HDM administration. Bronchoalveolar lavage fluid (BALF) was collected. Inflammatory cells in BALF were counted. Mucus production in lung tissues was evaluated by periodic acid Schiff (PAS) staining. Intraperitoneal (i.p.) and intranasal (IN) injection of HDM induced infiltration of inflammatory cells into lungs of mice. The increase of total cells was higher in young mice than in old mice. Mucus secretion from goblet cells increased after HDM administration, with young mice showing higher mucus production than old mice. In chronic asthmatic state induced by i.p. and IN (three times) injection, old mice exhibited more inflammatory cells, specifically eosinophils, and mucus production than young mice. Our findings suggest that age and exposure duration are critical factors in different manifestations of asthma. Thus, they should be overarchingly considered in drug development for asthma.

Keywords

Acknowledgement

This paper was supported by Wonkwang Health Science University in 2024.

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