Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Uncovering Candidate Pathogenicity Genes in Erwinia pyrifoliae YKB12327 via Tn5-insertion Mutagenesis

  • Hualin Nie (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Mi-Hyun Lee (Crop Protection Division, National Institute of Agricultural Science) ;
  • Sanghee Lee (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Seo-Rin Ko (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Young-Soo Hong (KRIBB School of Bioscience, Korea University of Science and Technology (UST)) ;
  • Jae Sun Moon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jun Myoung Yu (Department of Applied Biology, Chungnam National University) ;
  • Ah-Young Shin (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Suk-Yoon Kwon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2024.05.29
  • Accepted : 2024.07.26
  • Published : 2024.09.30
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Abstract

Erwinia pyrifoliae is a gram-negative bacterial pathogen that commonly causes black shoot blight in pear and apple tree. Although the pathogenicity of this bacterial species is very similar to E. amylovora, there is no specific explanation of its pathogenic genes and mechanisms. In this study, our investigation into E. pyrifoliae pathogenicity involved generating seven YKB12327 mutant strains using Tn5 transposon mutagenesis. Observations revealed weakened growth rate and loss of pathogenicity in these mutants. Whole-genome sequencing and alignment analysis identified transposon insertions within the coding sequences of five strains and in the intergenic region of two strains. Annotation analysis elucidated genes directly or indirectly associated with pathogenicity. Notably, mutant strain MT16 displayed a transposon insertion mutation in the cyclic-di-GMP phosphodiesterase (pdeF) gene, a key player in bacterial signaling, governing microbial behavior and adaptation to environmental changes. Our findings provide insights into the genetic regulation of E. pyrifoliae pathogenicity, suggesting potential avenues for further research aimed at understanding and controlling this bacterial pathogen by targeting pdeF to mitigate apple black shoot blight disease.

Keywords

Acknowledgement

This work was supported by grants from the Agenda program (PJ015594) of the Rural Development Administration and the Korean Research Institute of Bioscience and Biotechnology Initiative Program. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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