Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Investigation of bacterial diversity and analysis of pathogenic bacteria in wild boar nasal microbiota

멧돼지 비강 미생물군의 세균 다양성 조사 및 병원성 세균 분석

  • Chang-Gi Jeong (College of Veterinary Medicine, Jeonbuk National University) ;
  • Young-Sun Sung (College of Veterinary Medicine, Jeonbuk National University) ;
  • Jun-Soo Park (College of Veterinary Medicine, Jeonbuk National University) ;
  • Eun-Jee Na (College of Veterinary Medicine, Jeonbuk National University) ;
  • Su-Beom Chae (College of Veterinary Medicine, Jeonbuk National University) ;
  • Serin Sim (College of Veterinary Medicine, Jeonbuk National University) ;
  • Sun-Young Kim (College of Veterinary Medicine, Jeonbuk National University) ;
  • Young-Sik Kim (College of Veterinary Medicine, Jeonbuk National University) ;
  • Jae-Ku Oem (College of Veterinary Medicine, Jeonbuk National University)
  • 정창기 (전북대학교 수의과대학) ;
  • 성영선 (전북대학교 수의과대학) ;
  • 박준수 (전북대학교 수의과대학) ;
  • 나은지 (전북대학교 수의과대학) ;
  • 채수범 (전북대학교 수의과대학) ;
  • 심세린 (전북대학교 수의과대학) ;
  • 김선영 (전북대학교 수의과대학) ;
  • 김용식 (전북대학교 수의과대학) ;
  • 엄재구 (전북대학교 수의과대학)
  • Received : 2024.08.27
  • Accepted : 2024.09.04
  • Published : 2024.09.30
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Abstract

This study investigated the bacterial diversity and pathogenic bacteria in the nasal microbiota of wild boars (Sus scrofa) in South Korea, focusing on their potential role as reservoirs for pathogens. A total of 252 nasal swab samples were collected from wild boars in Namwon-si and Muju-gun between November 2023 and May 2024. The samples were analyzed using 16S rRNA sequencing and culture methods. Thirty-six bacterial species were identified, including 13 pathogenic species such as Streptococcus suis, Mycoplasma hyopneumoniae, Staphylococcus aureus, and Escherichia fergusonii. The presence of these pathogens suggests that wild boars may contribute to the spread of zoonotic diseases, posing risks to both livestock and human health. This study provides fundamental data for developing effective disease control and novel insight into nasal microbiota in wild boar in South Korea.

Keywords

References

  1. Agata N, Ohta M, Yokoyama K. 2002. Production of Bacillus cereus emetic toxin (cereulide) in various foods. Int J Food Microbiol 73: 23-27.
  2. Angen O, Quirie M, Donachie W, Bisgaard M. 1999. Investigations on the species specificity of Mannheimia (Pasteurella) haemolytica serotyping. Vet Microbiol 65: 283-290.
  3. Austin B, Austin DA. 2016. Bacterial fish pathogens: disease of farmed and wild fish. Springer. 
  4. Chambers HF, DeLeo FR. 2009. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 7: 629-641.
  5. Dallaire-Dufresne S, Tanaka KH, Trudel MV, Lafaille A, Charette SJ. 2014. Virulence, genomic features, and plasticity of Aeromonas salmonicida subsp. salmonicida, the causative agent of fish furunculosis. Vet Microbiol 169: 1-7.
  6. Duarte RS, Barros RR, Facklam RR, Teixeira LM. 2005. Phenotypic and genotypic characteristics of Streptococcus porcinus isolated from human sources. J Clin Microbiol 43: 4592-4601.
  7. Ehling-Schulz M, Lereclus D, Koehler TM. 2019. The Bacillus cereus group: Bacillus species with pathogenic potential. Microbiol Spectr 7: 10.1128/microbiolspec. gpp1123-0032-2018.
  8. Fano E, Pijoan C, Dee S. 2005. Dynamics and persistence of Mycoplasma hyopneumoniae infection in pigs. Can J Vet Res 69: 223.
  9. Farmer JJ 3rd, Fanning GR, Davis BR, O'hara CM, Riddle C, Hickman-Brenner FW, Asbury MA, Lowery VA 3rd, Brenner DJ. 1985. Escherichia fergusonii and Enterobacter taylorae, two new species of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol 21: 77-81.
  10. Fritzemeier J, Teuffert J, Greiser-Wilke I, Staubach C, Schluter H, Moennig V. 2000. Epidemiology of classical swine fever in Germany in the 1990s. Vet Microbiol 77: 29-41.
  11. Gortazar C, Ferroglio E, Hofle U, Frolich K, Vicente J. 2007. Diseases shared between wildlife and livestock: a European perspective. Eur J Wildlife Res 53: 241-256.
  12. Granum PE, Lund T. 1997. Bacillus cereus and its food poisoning toxins. FEMS Microbiol Lett 157: 223-228.
  13. Gursul NC, Ozdemir E. 2022. Streptococcus porcinus endocarditis: the first reported case in humans. Eskisehir Med J 3: 1-4.
  14. Igbinosa IH, Igumbor EU, Aghdasi F, Tom M, Okoh AI. 2012. Emerging Aeromonas species infections and their significance in public health. Sci World J 2012: 625023.
  15. Janda JM, Abbott SL. 2010. The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev 23: 35-73.
  16. Jo YS, Gortazar C. 2021. African swine fever in wild boar: assessing interventions in South Korea. Transbound Emerg Dis 68: 2878-2889.
  17. Ju DU, Jung K, Ohk CS, Kim SS, Ahn GH. 2020. A survey for prevalence of infectious diseases in wild boar (Sus scrofa) in northern Gyeonggi province, South Korea. Korean J Vet Serv 43: 155-159.
  18. Keuling O, Stier N, Roth M. 2008. How does hunting influence activity and spatial usage in wild boar Sus scrofa L.? Eur J Wildlife Res 54: 729-737.
  19. Kim YK, Lim SI, Kim JJ, Cho YY, Song JY, Cho IS, Hyun BH, Choi SH, Kim SH, Park EH, An DJ. 2016. Surveillance of classical swine fever in wild boar in South Korea from 2010-2014. J Vet Med Sci 77: 1667-1671.
  20. Liu J, Gao S, Dong Y, Lu C, Liu Y, 2020. Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila. BMC Microbiol 20: 1-13.
  21. Logan NA, Vos PD. 2015. Bacillus. Bergey's manual of systematics of archaea and bacteria, 1-163.
  22. Lowy FD. 1998. Staphylococcus aureus infections. N Engl J Med 339: 520-532.
  23. Lun ZR, Wang QP, Chen XG, Li AX, Zhu XQ. 2007. Streptococcus suis: an emerging zoonotic pathogen. Lancet Infect Dis 7: 201-209.
  24. Maheux A, Boudreau D, Bergeron M, Rodriguez M. 2014. Characterization of Escherichia fergusonii and Escherichia albertii isolated from water. J Appl Microbiol 117: 597-609.
  25. Manos J, Belas R. 2006. The genera proteus, providencia, and morganella. Prokaryotes 6: 245-269.
  26. Massei G, Roy S, Bunting R. 2011. Too many hogs? A review of methods to mitigate impact by wild boar and feral hogs. Human-Wildlife Interactions 5: 79-99.
  27. Menanteau-Ledouble S, Kumar G, Saleh M, El-Matbouli M. 2016. Aeromonas salmonicida: updates on an old acquaintance. Dis Aquat Organ 120: 49-68.
  28. Miller RA, Beno SM, Kent DJ, Carroll LM, Martin NH, Boor KJ, Kovac J. 2016. Bacillus wiedmannii sp. nov, a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments. Int J Syst Evol Microbiol 66: 4744-4753.
  29. Minana-Galbis D, Farfan M, Fuste MC, Loren JG. 2004. Aeromonas molluscorum sp. nov, isolated from bivalve molluscs. Int J Syst Evol Microbiol 54: 2073-2078.
  30. Nhinh DT, Le DV, Van KV, Huong Giang NT, Dang LT, Hoai TD. 2021. Prevalence, virulence gene distribution and alarming the multidrug resistance of Aeromonas hydrophila associated with disease outbreaks in freshwater aquaculture. Antibiotics 10: 532.
  31. NIBR. 2021. 2021 Wildlife Survey. 29-37.
  32. O'Hara CM, Brenner FW, Miller JM. 2000a. Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev 13: 534-546.
  33. O'Hara CM, Brenner FW, Steigerwalt AG, Hill BC, Holmes B, Grimont P, Hawkey PM, Penner JL, Miller JM, Brenner DJ. 2000b. Classification of Proteus vulgaris biogroup 3 with recognition of Proteus hauseri sp. nov, nom. rev. and unnamed Proteus genomospecies 4, 5 and 6. Int J Syst Evol Microbiol 50: 1869-1875.
  34. Raymond B, Federici BA. 2017. In defence of Bacillus thuringiensis, the safest and most successful microbial insecticide available to humanity-a response to EFSA. FEMS Microbiol Ecol 93: fix084.
  35. Savini V, Catavitello C, Talia M, Manna A, Pompetti F, Favaro M, Fontana C, Febbo F, Balbinot A, Di Berardino F. 2008. Multidrug-resistant Escherichia fergusonii: a case of acute cystitis. J Clin Microbiol 46: 1551-1552.
  36. Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler D, Dean D. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62: 775-806.
  37. Shewmaker PL, Steigerwalt AG, Whitney AM, Morey RE, Graziano JC, Facklam RR, Musser KA, Merquior VL, Teixeira LM. 2012. Evaluation of methods for identification and determination of the taxonomic status of strains belonging to the Streptococcus porcinus-Streptococcus pseudoporcinus complex isolated from animal, human, and dairy sources. J Clin Microbiol 50: 3591-3597.
  38. Venkataraman A, Bassis CM, Beck JM, Young VB, Curtis JL, Huffnagle GB, Schmidt TM. 2015. Application of a neutral community model to assess structuring of the human lung microbiome. MBio 6, 10.1128/mbio.02284-02214.
  39. Wang Y, Guo H, Bai Y, Li T, Xu R, Sun T, Lu J, Song Q. 2020. Isolation and characteristics of multi-drug resistant Streptococcus porcinus from the vaginal secretions of sow with endometritis. Bmc Vet Res 16: 1-8.
  40. Wong-Lee JG, Lovet M. 1993. Rapid and sensitive PCR method for identification of Mycoplasma species in tissue culture. In: Persing D, ed. Diagnostic molecular and microbiology principles and applications. Washington, DC: American Society for Microbiology; 1993: 257-260.