The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Molecular Identification and Genetic Diversity Analysis of Papaya Leaf Curl China Virus Infecting Ageratum conyzoides

  • Liping Zhang (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University) ;
  • Shujie Wu (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University) ;
  • Meisheng Zhao (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University) ;
  • Hussein Ghanem (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University) ;
  • Gentu Wu (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University) ;
  • Mingjun Li (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University) ;
  • Ling Qing (Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University)
  • Received : 2024.04.09
  • Accepted : 2024.07.18
  • Published : 2024.10.01
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Abstract

Papaya leaf curl China virus (PaLCuCNV) is a damaging plant pathogen causing substantial losses to crop. The complete genomes of three PaLCuCNV isolates from Ageratum conyzoides were obtained and combined with the 68 reference isolates in GenBank for comprehensive genetic diversity analyses using specialized computational tools. Sequence alignment revealed nucleotide sequence similarity ranging from 85.3% to 99.9% among 71 PaLCuCNV isolates. Employing phylogenetic analysis, 71 PaLCuCNV sequences were clustered into five groups, with no significant correlation observed between genetic differentiation and either host species or geographical origin. Additionally, 13 recombination events across all PaLCuCNV isolates were identified. Genetic diversity analysis indicated the ongoing expansion and evolution of PaLCuCNV populations, supported by a neutral model. Moreover, significant genetic differentiation was observed among distinct viral populations, primarily attributed to genetic drift. Overall, our findings provide valuable insights into the detection, genetic variation, and evolutionary dynamics of PaLCuCNV.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation of China (No. 31272013), the Fundamental Research Funds for the Central Universities of the Ministry of Education of China (No. XDJK2017A006), and the Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates (No. S202310635211 and No. 201610635053).

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