The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development and Validation of an SNP Marker for Identifying Xanthomonas oryzae pv. oryzae Thai Isolates That Break xa5-Mediated Bacterial Blight Resistance in Rice

  • Tebogo Balone (Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University) ;
  • Ananda Nuryadi Pratama (Center of Excellence in Research for Agricultural Biotechnology, Naresuan University) ;
  • Werapat Chansongkram (Center of Excellence in Research for Agricultural Biotechnology, Naresuan University) ;
  • Thanita Boonsrangsom (Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University) ;
  • Kawee Sujipuli (Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University) ;
  • Kumrop Ratanasut (Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University)
  • Received : 2024.04.30
  • Accepted : 2024.08.12
  • Published : 2024.10.01
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Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a pathogenic bacterium responsible for bacterial blight (BB) disease in rice, primarily mediated by the interaction between the plant and pathogen. The virulence mechanism involves the activation of the Sugars Will Eventually be Exported Transporter (SWEET) gene family in rice by transcription activator-like effectors derived from Xoo. The BB resistance gene xa5 has been identified as one of the most effective genes against Thai Xoo isolates, but xa5-mediated resistance-breaking Xoo strains have emerged. This study aimed to develop a single nucleotide polymorphism (SNP) marker for precise identification of xa5-mediated resistance-breaking Xoo. Comparative genomics of Thai Xoo isolates Xoo16PK001 and Xoo16PK002, which were incompatible and compatible with rice variety IRBB5 carrying xa5, respectively, identified eight SNP positions for the development of an SNP marker. The SNP marker XooE6 yields a specific 1,143 bp PCR product unique to Xoo16PK002. Screening 61 Thai isolates using XooE6 identified two positives: Xoo20PL010 and Xoo20UT002. Inoculation tests on rice varieties IRBB5 and IRBB13 demonstrated compatibility with IRBB5 and incompatibility with IRBB13, which bears Xa5 and xa13. Xoo16PK001 (XooE6-negative) showed different virulence. Inoculation on IRBB21 harboring Xa5, Xa13, and Xa21 resulted in partial resistance to both XooE6-positive and -negative strains. XooE6-positive strains up-regulated SWEET11 and suppressed SWEET14 in IRBB5, while Xoo16PK001 slightly induced SWEET11 but activated SWEET14 in IRBB13. This highlights the potential of XooE6 to identify xa5-mediated resistance-breaking Xoo strains and elucidate their pathogenic mechanisms through the upregulation of SWEET11.

Keywords

Acknowledgement

This work was supported by the Program Management Unit for Human Resources & Institutional Development, Research and Innovation (PMU-B), Thailand [grant number B05F640157]; Naresuan University [grant number R2566B027]; Naresuan University [grant number R2565B003]; and the Thailand International Cooperation Agency (TICA) scholarship.

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