The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Re-identification of Korean Isolates in the Colletotrichum dematium, C. magnum, C. orchidearum, and C. orbiculare Species Complexes

  • Le Dinh Thao (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyorim Choi (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Donghun Kang (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Anbazhagan Mageswari (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Daseul Lee (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Dong-Hyun Kim (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • In-Young Choi (Department of Agricultural Biology, Jeonbuk National University) ;
  • Hyeon-Dong Shin (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Seung-Beom Hong (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2024.05.24
  • Accepted : 2024.08.01
  • Published : 2024.10.01
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Abstract

A large number of species in the genus Colletotrichum have been reported as causal agents of anthracnose on crops and wild plants in Korea. Many Colletotrichum isolates from the country preserved in the Korean Agricultural Culture Collection (KACC) were previously identified based on host plants and morphological characteristics, and it may lead to species misidentification. Thus, accurate fungal species identification using multilocus sequence analyses is essential for understanding disease epidemiology and disease management strategies. In this study, combined DNA sequence analyses of internal transcribed spacer, gapdh, chs-1, his3, act, tub2, and gs were applied to re-identify 27 Colletotrichum isolates in KACC. The phylogenetic analyses showed that the isolates resulted in 11 known species, they belong to the C. dematium species complex (C. hemerocallidis, C. jinshuiense, and C. spinaciae), the C. magnum complex (C. kaifengense and C. cf. ovatense), the C. orchidearum complex (C. cattleyicola, C. plurivorum, C. reniforme, and C. sojae) and the C. orbiculare complex (C. malvarum and C. orbiculare). Of them, C. cattleyicola, C. hemerocallidis, C. kaifengense, and C. reniforme were unrecorded species in Korea. In the view of host-fungus combinations, 10 combinations are newly reported in the world and 12 are new reports in Korea, although their pathogenicity on the host was not confirmed.

Keywords

Acknowledgement

This study was supported by the grant (PJ017286) from the Rural Development Administration and the grant (M3H9A1081254) from the Ministry of Science and ICT in Korea. We are sincerely thankful to Nan-Hee Lee, Seon-Hee Kim, and Eun-Ha Yuk for their laboratory assistance.

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