International Journal of Stem Cells

Korean Society for Stem Cell Research (한국줄기세포학회)
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Establishing Three-Dimensional Explant Culture of Human Dental Pulp Tissue

  • Eun Jin Seo (Dental and Life Science Institute, School of Dentisty, Pusan National University) ;
  • Soyoung Park (Dental and Life Science Institute, School of Dentisty, Pusan National University) ;
  • Eungyung Lee (Dental and Life Science Institute, School of Dentisty, Pusan National University) ;
  • Yang Hoon Huh (Center for Electron Microscopy Research, Korea Basic Science Institute) ;
  • Ye Eun Ha (Tissue Regeneration Laboratory, StemDen Co., Ltd.) ;
  • Gabor J. Tigyi (Department of Physiology, The University of Tennessee Health Science Center) ;
  • Taesung Jeong (Dental and Life Science Institute, School of Dentisty, Pusan National University) ;
  • Il Ho Jang (Dental and Life Science Institute, School of Dentisty, Pusan National University) ;
  • Jonghyun Shin (Dental and Life Science Institute, School of Dentisty, Pusan National University)
  • Received : 2023.06.30
  • Accepted : 2024.04.15
  • Published : 2024.08.30
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Abstract

Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth, the intact tissue was placed between two layers for Matrigel with addition of the culture medium. The reticular outgrowth of pre-odontoblast layer continued for a month and the random accumulation of dentin was observed near the end. Electron microscopy showed the cellular organization and in situ development of dentin, and immunohistochemistry exhibited the expression of odontoblast and stem cell markers in the outgrowth area. Three-dimensional explant culture of human dental pulp will provide a novel platform for understanding stem cell biology inside the tooth and developing the regenerative medicine.

Keywords

Acknowledgement

This study was supported by Dental Research Institute (PNUDHDRI-2020-05), Pusan National University Dental Hospital.

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