Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Honey and levodopa comparably preserved substantia nigra pars compacta neurons through the modulation of nuclear factor erythroid 2-related factor 2 signaling pathway in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model

  • Fatimo Ajoke Sulaimon (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Ruqayyah Yetunde Ibiyeye (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Kwara State University) ;
  • Aminu Imam (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Aboyeji Lukuman Oyewole (Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Abubakar Lekan Imam (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Monsur Shehu (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Sikiru Abayomi Biliaminu (Department of Chemical Pathology, Faculty of Basic Clinical Sciences, College of Health Sciences, University of Ilorin) ;
  • Risikat Eniola Kadir (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Gabriel Olaiya Omotoso (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin) ;
  • Moyosore Salihu Ajao (Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin)
  • Received : 2024.02.05
  • Accepted : 2024.05.09
  • Published : 2024.09.30
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Abstract

Parkinson's disease (PD) affects about 8.5 million individuals worldwide. Oxidative and inflammatory cascades are implicated in the neurological sequels, that are mostly unresolved in PD treatments. However, proper nutrition offers one of the most effective and least costly ways to decrease the burden of many diseases and their associated risk factors. Moreover, prevention may be the best response to the progressive nature of PD, thus, the therapeutic novelty of honey and levodopa may be prospective. This study aimed to investigate the neuroprotective role of honey and levodopa against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced oxidative stress. Fifty-four adult male Swiss mice were divided into control and PD model groups of 27 mice. Each third of the control mice either received phosphate buffered saline, honey, or levodopa for 21 days. However, each third of the PD models was either pretreated with honey and levodopa or not pretreated. Behavioral studies and euthanasia were conducted 2 and 8 days after MPTP administration respectively. The result showed that there were significantly (P<0.05) higher motor activities in the PD models pretreated with the honey as well as levodopa. furthermore, the pretreatments protected the midbrain against the chromatolysis and astrogliosis induced by MPTP. The expression of antioxidant markers (glutathione [GSH] and nuclear factor erythroid 2-related factor 2 [Nrf2]) was also significantly upregulated in the pretreated PD models. It is thus concluded that honey and levodopa comparably protected the substantia nigra pars compacta neurons against oxidative stress by modulating the Nrf2 signaling molecule thereby increasing GSH level to prevent MPTP-induced oxidative stress.

Keywords

Acknowledgement

We hereby acknowledge the Dr. Ijomone, O.M of Neurostein Lab, Ondo, Adeyemo E.A, Emaye M.A and Yussuf R.A. Olaoye J, Jimoh H.B, and Ashir F, for their technical support. The technical team of the central research laboratory, Tanke is also well-appreciated.

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