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Morphological aspects of small intestinal mucosal injury and repair after electron irradiation

  • Grigory Demyashkin (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Elza Karakaeva (National Medical Research Centre of Radiology, Ministry of Health of Russia) ;
  • Siuzanna Saakian (National Medical Research Centre of Radiology, Ministry of Health of Russia) ;
  • Vladimir Shchekin (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Emir Elbuzdukaev (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Umar Bamatgiraev (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Daniia Ashgalieva (Federal State Budget Educational Institution of Higher Education A.I. Yevdokimov Moscow State University of Medicine and Dentistry (MSUMD)) ;
  • Makka Evsultanova (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Daniil Kovalev (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Darya Kabanova (Federal State Budget Educational Institution of Higher Education A.I. Yevdokimov Moscow State University of Medicine and Dentistry (MSUMD)) ;
  • Oleg Shatunov (Laborant of the Labrotory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)) ;
  • Dmitrii Atiakshin (Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University)
  • 투고 : 2024.02.21
  • 심사 : 2024.04.12
  • 발행 : 2024.09.30

초록

Morphological evaluation of the small intestine mucosa and apoptosis activity (caspase-3) is necessary to assess the severity of damage to the small intestine. At the same time, proliferative index based on Ki-67 can be used to assess the regenerative potential of the small intestine. Fragments of small intestine of Wistar rats (n=60) of three groups: I) control (n=20); II) experimental group (n=20; local single electron irradiation at a dose of 2 Gy), III) experimental group (n=20; local single electron irradiation at a dose of 8 Gy) were studied by light microscopy using hematoxylin and eosin staining and immunohistochemical reactions with antibodies to Ki-67 and caspase-3. In all samples of the experimental groups, a decrease in all morphometric indices was observed on day 1 with a tendency to recover on day 3. Small intestinal electron irradiation led to disturbances in the histoarchitecture of varying severity, and an increase in cell apoptosis was observed (increased expression of caspase-3 and decrease in Ki-67). In addition, modulation of the PI3K/AKT and MAPK/ERK signaling pathways was detected. The most pronounced destructive changes were observed in the group of 8 Gy single electron irradiation. Local irradiation of the small intestine with electrons at a dose of 2 and 8 Gy results in a decrease in the number of enterocytes, mainly stem cells of the intestinal crypts.

키워드

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