한국버섯학회지 (Journal of Mushroom)

  • 제22권3호
  • /
  • Pages.92-101
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  • 2024
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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꽃송이버섯 추출물이 함유된 헤어 트리트먼트의 손상 모발 개선효과

Treatment with Sparassis latifolia extract for repairing damaged hair

  • 최현아 (조선대학교 산업대학원 미용향장학과) ;
  • 이여진 (조선대학교 대학원 화학공학과) ;
  • 최문희 (주식회사 섬섬바이오) ;
  • 신현재 (조선대학교 산업대학원 미용향장학과)
  • Hyun-A Choi (Department of Beauty and Cosmetology, Graduate School of Industrial Technology and Entrepreneurship, Chosun University) ;
  • Yeo-Jin Lee (Department of Chemical Engineering, Graduate School of Chosun University) ;
  • Moon-Hee Choi (Research institute affiliated, SUMSUMBIO Co., Ltd.) ;
  • Hyun-Jae Shin (Department of Beauty and Cosmetology, Graduate School of Industrial Technology and Entrepreneurship, Chosun University)
  • 투고 : 2024.06.24
  • 심사 : 2024.08.21
  • 발행 : 2024.09.30
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초록

꽃송이버섯 추출물과 분획물을 제조하여 항산화 활성 평가 및 HPLC, LC-MS/MS를 이용하여 폴리페놀 함량을 분석하였다. 또한, 꽃송이버섯 추출물을 함유한 트리트먼트를 제조하여 모발의 굵기, 인장강도 및 SEM 측정을 통해 손상된 모발개선 효과를 평가하였다. DPPH 및 ABTS 라디칼 소거능 측정결과, 열수 추출물의 IC50은 각각 2.49 mg/mL와 1.61 mg/mL, EtOAc 분획물의 IC50은 각각 0.97 mg/mL와 0.48 mg/mL로 확인하였다. HPLC 및 LC-MS/MS 분석에서 EtOAc 분획물에 4-hydroxybenzoic acid, gallic acid, benzoic acid가 각각 4,550 ㎍/g, 1,828.8 ㎍/g, 2,427.56 ㎍/g 및 652 ㎍/g, 468 ㎍/g, 2,170 ㎍/g로 확인되었다. 70% EtOH 추출물을 포함한 트리트먼트 처리군에서 모발의 굵기는 손상된 실험군에 비해 22.58%, 인장강도는 38.77% 증가하였고, SEM 측정 결과 큐티클 구조가 규칙적이고 균일한 형태를 보여 모발 손상이 회복된 모습을 확인하였다. 본 연구 결과는 꽃송이버섯 추출물이 다양한 폴리페놀을 포함하여 항산화 활성 및 모발 개선에 효과가 있어 화장품 및 식품 등 모발 보호 제품 개발의 기초 자료로 활용될 수 있다. 추후 모유두 세포를 통한 항산화 효과와 모발 성장 인자 단백질 발현 측정으로 모낭 주기 조절 메커니즘을 규명하고, 임상적으로 모발의 탄력과 윤기 변화를 검증하는 추가 연구가 필요하다.

Hair dyeing, hair perming, and daily hair dryer use can substantially damage hair. Consequently, the demand for products containing natural ingredients for the care of damaged hair is growing. Although polyphenols with antioxidant effects are often used for hair conditioning, few studies have focused on hair conditioning, and the potential of Sparassis latifolia mushroom extract for hair improvement has not been evaluated to date. In this study, the antioxidant activity of and polyphenol content in hot water, 70% and 100% ethanol (EtOH), n-hexane, ethyl acetate (EtOAc), and water extracts of S. latifolia were analyzed. A hair treatment containing S. latifolia extract was prepared, and its effect on damaged hair was evaluated. The highest antioxidant activity was observed in the hot water and EtOAc extracts. Moreover, polyphenol analysis using high-performance liquid chromatography and liquid chromatography-mass spectroscopy confirmed that the EtOAc fraction has relatively high contents of specific polyphenols beneficial for hair. Based on these results, a hair treatment containing S. latifolia extract was applied to damaged hair, and hair improvement was evaluated using hair thickness, tensile strength, and scanning electron microscopy. The hair treatment containing 70% EtOH extract effectively improved hair condition. We postulate that this improvement was caused by the high hydrophobic polyphenol content in the 70% EtOH extract.

키워드

과제정보

본 연구는 과학기술정보통신부의 재원으로 한국연구재단(No. 2017R1A2B4006204)의 지원을 통해 수행되었습니다.

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