Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Morphological and molecular identification of Metopograpsus crab caught from the coast of Tambala Village, North Sulawesi, Indonesia

  • Darus Sa'adah Johanis Paransa (Marine Science, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University) ;
  • Kurniati Kemer (Marine Science, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University) ;
  • Rene Charles Kepel (Fisheries Resources Management, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University) ;
  • Desy Helena Maria Mantiri (Marine Science, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University) ;
  • Beivy Jonathan Kolondam (Department of Biology, Faculty of Mathematics and Natural Science, Sam Ratulangi University)
  • Received : 2023.11.16
  • Accepted : 2024.02.04
  • Published : 2024.07.31
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Abstract

The objective of this research was to identify the morphology and molecular profile of crab caught from the coast of Tambala Village, Tombariri District, Minahasa Regency, North Sulawesi. Crabs were collected from the intertidal zone at night during the lowest tide. Crabs were morphological identified by description of shape, color and size of the carapace. Molecular identification was done through DNA barcoding including DNA extraction, amplification of the cytochrome c oxidase subunit I (COI) gene and electrophoresis. Morphological and genetic analysis identified the crab species as Metopograpsus oceanicus.

Keywords

Acknowledgement

We would like to appreciate Ms. Adrian for crab sampling activities. Our thanks are also addressed to Ms. Mokoginta F.S and Ms. Jofanka L. for their laboratory help.

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