Journal of Animal Science and Technology

  • 제66권5호
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  • Pages.936-948
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  • 2024
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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Evaluation of conical 9 well dish on bovine oocyte maturation and subsequent embryonic development

  • Byung Hyun Ju (Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University) ;
  • You Jin Kim (Department of Obstetrics & Gynecology, Chungnam National University Hospital) ;
  • Youn Bae Park (Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University) ;
  • Byeong Ho Kim (Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University) ;
  • Min Kyu Kim (Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University)
  • 투고 : 2024.05.10
  • 심사 : 2024.07.01
  • 발행 : 2024.09.30
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초록

The Conical 9 well dish (C9 well dish) is characterized by a decreasing cross-sectional area towards the base. This design was hypothesized to enhance embryonic development by emulating the in vivo physical environment through density modulation. Comparative analyses revealed no significant difference in nuclear maturation rates between the C9 well dish and the 5-well dish. Reactive oxygen species (ROS) generation was lower in the C9 well dish compared to the 5-well dish; however, this difference was not statistically significant. On the second day of in vitro culture, the cleavage rate in the C9 well dish was 4.66% higher, although not statistically significant, and the rates of blastocyst development were similar across both dishes. No significant differences were observed in the intracellular levels of glutathione (GSH) and ROS, as well as in the total cell number within the blastocysts between the dish types. The expression of mitogen-related factors, TGFα and IGF-1, in the blastocysts was consistent between the dishes. However, PDGFβ expression was significantly lower in the C9 well dish compared to the 35 mm petri dish. Similarly, the expression of the apoptosis factor Bax/Bcl2l2 showed no significant differences between the two dishes. Despite the marked difference in PDGFβ expression, its impact on blastocyst formation appeared negligible. The study also confirmed the feasibility of culturing a small number of oocytes per donor, collected via Ovum Pick-Up (OPU), with reduced volumes of culture medium and mineral oil, thus offering economic advantages. In conclusion, the present study indicates that the C9 well dish is effective for in vitro development of a small quantity of oocytes and embryos, presenting it as a viable alternative to traditional cell culture dishes.

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과제정보

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant number : HI22C1754).

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