Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Complete genome sequence of candidate probiotic Limosilactobacillus fermentum KUFM407

  • Bogun Kim (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Ji yu Heo (Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Xiaoyue Xu (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Hyunju Lee (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Duleepa Pathiraja (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Jae-Young Kim (Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Yi Hyun Choi (Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • In-Geol Choi (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Sae Hun Kim (Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University)
  • Received : 2023.08.21
  • Accepted : 2023.10.31
  • Published : 2024.07.31
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Abstract

It has been reported that the administration of Limosilactobacillus fermentum alleviates diseases such as osteoporosis and colitis. In this study, we report the complete genome sequence of Limosilactobacillus fermentum KUFM407, a probiotic strain of LAB isolated from Korean traditional fermented food, Kimchi. Whole genome sequencing of L. fermentum KUFM407 was performed on the Illumina MiSeq and Oxford Nanopore MinION platform. The genome consisted of one circular chromosome (2,077,616 base pair [bp]) with a guanine cytosine (GC) content of 51.5% and one circular plasmid sequence (13,931 bp). Genome annotation identified 1,932 protein-coding genes, 15 rRNAs, and 58 tRNAs in the assembly. The function annotation of the predicted proteins revealed genes involved in the biosynthesis of bacteriocin and fatty acids. The complete genome of L. fermentum KUFM407 could provide valuable information for the development of new probiotic food and health supplements.

Keywords

Acknowledgement

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through High Valueadded Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321034053HD020, 1545027002) and supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries, funded by the Ministry of Agriculture, Food, and Rural Affairs (32136-05-1-SB010).

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