Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of Polysaccharide (Polycan) derived from Black Yeast in TNF-α-induced Inflammation in the Intestinal Epithelial Cells and Loperamide-induced Constipation Models

흑효모 유래 Polycan의 TNF-α 유도 장 상피세포 염증 및 Loperamide 유도 변비 모델에 미치는 효과

  • 김영숙 ((주)글루칸) ;
  • 구본화 ((주)글루칸) ;
  • 조민정 ((주)글루칸) ;
  • 권정희 ((주)엔피켐바이오) ;
  • 이선민 (안전성평가연구소 경남분소 경남바이오헬스연구지원센터) ;
  • 오태우 (한국한의학연구원 한의기술응용센터)
  • Received : 2024.08.20
  • Accepted : 2024.08.30
  • Published : 2024.08.31
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Abstract

Objective : This study was conducted to investigate the anti-inflammatory and laxative effects of Polycan in TNF-α-treated HT-29 intestinal epithelial cells and loperamide-induced constipation in vivo models, respectively. Methods : To evaluate the anti-inflammatory effects of Polycan, HT-29 cells were treated with TNF-α in the presence or absence of Polycan. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA). MAPK phosphorylation, nuclear translocation of NF-κB, and phosphorylation of IκB were assessed by Western blot analysis. To investigate the laxative effects of Polycan, 6-week-old SD rats (8 female rats per group) were orally administered Polycan or Chicory Fiber as a positive control for 4 weeks, and constipation was induced with loperamide treatment for 10 days before sacrifice. One day before sacrifice, a charcoal meal was administered to evaluate intestinal transit times. The periodically collected feces were used to assess the number of fecal pellets and fecal water content. Results : Polycan inhibited TNF-α-induced IL-8 expression in dose-dependent manner. Furthermore, Polycan suppressed TNF-α-induced phosphorylation of MAPKs (ERK1/2, p38 and JNK), degradation of Iκ-Bα and nuclear translocation of NF-κB. In an in vivo constipation model, the number of fecal pellets per food intake was significantly increased in rats administered with Polycan, both 1 day and 7 days after loperamide treatment. The water content of fecal pellets was restored in the Polycan groups starting 7 days after loperamide treatment. In addition, Polycan intake significantly enhanced the gastrointestinal transit ratio of a charcoal meal but reduced the number of intestinal fecal pellets. Conclusions : These results suggest that Polycan suppressed TNF-α-induced inflammation by blocking both the MAPK and NF-κB pathways in HT-29 cells. Additionally, in a loperamide-induced constipation model, Polycan showed clear laxative effects by increasing the number of fecal pellets, fecal water content, and intestinal transit ratio of a charcoal meal.

Keywords

Acknowledgement

이 연구는 산업통상자원부, 한국산업기술진흥원(KIAT)의 "바이오활성제제 글로벌 경쟁력강화사업 (과제번호:B0080207002074)의 지원을 받아 수행되었습니다.

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