Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Preventive effects of Scutellaria baicalensis, Alisma orientale and Atractylodes japonica mixtures extract on Interleukin-6-induced intestinal tight junction dysfunction in Caco-2 cells

Caco-2 세포에서 Interleukin-6로 유도한 밀착연접 기능 장애에 대한 황금, 택사, 백출 혼합 추출물의 예방 효과

  • Kwang-Youn Kim (Korean Medicine Application Center, Korea Institute of Oriental Medicine) ;
  • Tae-In Kim (Korean Medicine Application Center, Korea Institute of Oriental Medicine) ;
  • Yeon-Ji Kim (Korean Medicine Application Center, Korea Institute of Oriental Medicine) ;
  • Yun-Mi Kang (Korean Medicine Application Center, Korea Institute of Oriental Medicine) ;
  • Kyungho Kim (Korean Medicine Application Center, Korea Institute of Oriental Medicine)
  • 김광연 (한국한의학연구원 한의기술응용센터) ;
  • 김태인 (한국한의학연구원 한의기술응용센터) ;
  • 김연지 (한국한의학연구원 한의기술응용센터) ;
  • 강윤미 (한국한의학연구원 한의기술응용센터) ;
  • 김경호 (한국한의학연구원 한의기술응용센터)
  • Received : 2024.03.19
  • Accepted : 2024.05.23
  • Published : 2024.05.31
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Abstract

Objectives : This study aimed to investigate the protective effects of mixed extracts from Scutellaria baicalensis Georgi, Alisma orientale Juzepzuk, and Atractylodes japonica Koidzumi on interleukin (IL)-6-induced damage to tight junction (TJ) integrity in a Caco-2 cell model. Methods : We assessed the TJ integrity of Caco-2 monolayers by measuring the flux of FITC-labeled dextran and transepithelial electrical resistance (TEER). Additionally, we evaluated the expression of TJ proteins, such as ZO-1 and Occludin. Results : Treatment with IL-6 (50 ng/ml) increased TJ permeability and decreased TEER values of Caco-2 monolayers. Pretreatment with HTB (50-200 ㎍/ml) for 1 h significantly alleviated IL-6-induced TJ disruption, as evidenced by reduced TJ permeability and increased TEER values. Furthermore, HTB reversed the IL-6-induced inhibition of TJ protein expression, including ZO-1 and Occludin. Conclusions : These findings indicate that HTB protects barrier function by reversing the IL-6-induced decrease in TJ integrity and the suppression of TJ protein expression.

Keywords

Acknowledgement

이 논문은 2021년 대한민국 교육부와 한국연구재단의 지원을 받아 수행된 연구임(NRF-2021R1I1A2040301).

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