한국유기농업학회지 (Korean Journal of Organic Agriculture)

  • 제32권3호
  • /
  • Pages.277-288
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  • 2024
  • /
  • 1229-3571(pISSN)
  • /
  • 2287-819X(eISSN)
한국유기농업학회 (Korean Association of Organic Agriculture)
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국내 자생 해조류 첨가가 in vitro 반추위 발효 성상 및 메탄 발생량에 미치는 영향

The Effects of Korean Seaweeds on In vitro Ruminal Fermentation Characteristics and Methane Production

  • 김별 (농촌진흥청 국립축산과학원 동물영양생리과) ;
  • 위지수 (농촌진흥청 국립축산과학원 동물영양생리과) ;
  • 이유경 (농촌진흥청 국립축산과학원 동물영양생리과) ;
  • 김현상 (농촌진흥청 국립축산과학원 동물영양생리과) ;
  • 성필남 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 이성대 (농촌진흥청 국립축산과학원 동물영양생리과) ;
  • 황일기 (해양수산부 국립수산과학원 수산식물품종관리센터) ;
  • 김현철 (해양수산부 국립수산과학원 육종연구센터) ;
  • 이성신 (농촌진흥청 국립축산과학원 동물영양생리과)
  • 투고 : 2024.03.26
  • 심사 : 2024.07.02
  • 발행 : 2024.08.30
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초록

국내에서 자생하고 있는 해조류를 첨가하여 in vitro 반추위 발효성상 및 메탄 발생량 분석을 실시하였다. 해조류 처리구에서 in vitro 배양 24시간대 총 가스 및 메탄 발생량 결과는 전 처리구에서 유의적인 차이가 없었으나 배양 48시간대 메탄 발생량 결과에서는 누른 끈적이와 청각 처리구에서 유의적으로(p<0.05) 낮았다. 해조류 처리구의 반추위 pH는 5.75~6.12로 대조구 대비 유의적으로(p<0.05) 낮았다. Total VFA 농도는 배양 24시간대에서 유의적인 차이가 없었으나, 청각 처리구에서 배양 48시간대 대조구 대비 유의적으로(p<0.05) 높았다. NH3-N 농도는 톳 처리구에서 배양 24시간과 48시간대 모두 대조구 대비 유의적으로 (p<0.05) 낮았다. 건물 소화율은 배양 24시간과 48시간대 모두 유의적인 차이를 보이지 않 아 해조류가 반추위 건물 소화율에 부정적인 영향을 미치지 않았다. 이를 통해 청각이 반추위 발효성상 및 소화율에 부정적인 영향을 미치지 않으면서 메탄 발생량을 감소시키는 효과를 보여, 추후 메탄 저감 소재로서 평가를 위해 가축 급여 실험을 통한 검증이 필요하다.

The present study was conducted to investigate the effects of seaweeds on in vitro ruminal fermentation characteristics and methane gas production. Five seaweeds (Dictyota dichotoma, DD; Chrysymenia wrightii Yamada, CW; Codium fragile, CF; Sargassum fusiforme, SF; Gracilaria vermiculophylla, GV) were obtained from National Institute of Fisheries Science (NIFS) in South Korea. The ruminal fluids were collected from 3 rumen-cannulated Hanwoo steers (average 12-months-old). The buffered ruminal fluids (50 mL) were incubated with substrates (0.4 g of concentrate and 0.1 g of rice straw in dry matter basis) and seaweeds (5% of substrates) at 39℃ for 24 and 48 hours. The total gas and methane production of all treatments incubated for 24 hours were not affected by the seaweed. However, methane production (mL/g of digested dry matter) in the CW and CF treatments incubated for 48 hours was decreased compared to control (p<0.05). Additionally, the ruminal pH of all treatments incubated for 24 and 48 hours was lower than control (p<0.05). There was no signigicant difference in total VFA concentration at 24 hours of incubation, but it was higher in the CF treatment at 48 hours of incubation (p<0.05). The dry matter digestibility of all treatments incubated for 24 and 48 hours were not affected by the seaweed. In conclusion, Codium fragile reduced in vitro methane production without negative effects on rumen fermentation characteristics.

키워드

과제정보

본 연구는 농촌진흥청 연구사업(RS-2023-00231243)의 지원에 의해 이루어진 것임.

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