한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제52권2호
  • /
  • Pages.218-220
  • /
  • 2024
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Complete Genome Sequence of Bifidobacterium longum subsp. longum DS0950 Isolated from Infant Feces with Obesity-Ameliorating Effects

  • Hana Jo (Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yong-Sik Kim (Department of Microbiology, College of Medicine, Soonchunhyang University) ;
  • Doo-Sang Park (Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2024.02.08
  • 심사 : 2024.04.03
  • 발행 : 2024.06.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Bifidobacterium longum subsp. longum DS0950 (B. longum DS0950)은 신생아 분변에서 분리되었으며 비만 개선 효능이 보고되었다. B. longum DS0950 전장유전체 서열은 PacBio RS II platforms을 이용하여 확보하였으며, 유전체의 크기는 2,433,092 bp의 단일 contig로 분석이 되었다. B. longum DS0950 균주는 박테리오신의 합성에 관련된 유전자와, ribulose-5-phosphate로부터 xylitol 생산할 수 있는 일련의 유전자를 보유하고 있다.

Bifidobacterium longum subsp. longum DS0950 (B. longum DS0950) was isolated from infant feces and has been reported to be effective in preventing obesity. The whole-genome sequence of B. longum DS0950 was obtained using the PacBio RS II platform, and it was consists of a single chromosome of 2,433,092 bp. The B. longum DS0950 contains genes associated with the synthesis of bacteriocins and a series of genes capable of producing xylitol from ribulose-5-phosphate.

키워드

과제정보

This work was carried out with the support of a Korea Innovation Foundation (INNPOLIS) grant (2021-DD-UP-0380-01) funded by the Korean government (the Ministry of Science and ICT) and a grant from the National Research Foundation of Korea (2022M3H9A1084279) funded by the Ministry of Science and ICT.

참고문헌

  1. Zhang C, Yu Z, Zhao J, Zhang H, Zhai Q, Chen W. 2019. Colonization and probiotic function of Bifidobacterium longum. J. Funct. Foods 53: 157-165. 
  2. Rahman MS, Lee Y, Park DS, Kim YS. 2023. Bifidobacterium bifidum DS0908 and Bifidobacterium longum DS0950 culture-supernatants ameliorate obesity-related characteristics in mice with high-fat diet-induced obesity. J. Microbiol. Biotechnol. 33: 96-105. 
  3. Yu Z, Morrison M. 2004. Improved extraction of PCR-quality community DNA from digesta and fecal samples. BioTechniques 36: 808-812. 
  4. Chin C-S, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, et al. 2013. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat. Methods 10: 563-569. 
  5. Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30: 2068-2069. 
  6. Malberg Tetzschner AM, Johnson JR, Johnston BD, Lund O, Scheutz F. 2020. In Silico genotyping of Escherichia coli isolates for extraintestinal virulence genes by use of whole-genome sequencing data. J. Clin. Microbiol. 58: e01269-20. 
  7. Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M, Edalatmand A, et al. 2019. CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res. 48: D517-D525. 
  8. Johansson MH, Bortolaia V, Tansirichaiya S, Aarestrup FM, Roberts AP, Petersen TN. 2021. Detection of mobile genetic elements associated with antibiotic resistance in Salmonella enterica using a newly developed web tool: Mobile element finder. J. Antimicrob. Chemother. 76: 101-109. 
  9. van Heel AJ, de Jong A, Song C, Viel JH, Kok J, Kuipers OP. 2018. BAGEL4: a user-friendly web server to thoroughly mine RiPPs and bacteriocins. Nucleic Acids Res. 46: W278-W281.