한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제52권2호
  • /
  • Pages.211-214
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  • 2024
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Draft Genome Sequence of Mycobacterium abscessus Treated with a Fluoroquinolone in a Time-Dependent Manner

  • Du-Gyeong Han (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Ji-A Jeong (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Sung-Kyoung Lee (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Seong-Han Kim (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency) ;
  • Se-Mi Jeon (Department of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Disease, Korea National Institute of Health, Korea Disease Control and Prevention Agency)
  • 투고 : 2024.03.12
  • 심사 : 2024.05.13
  • 발행 : 2024.06.28
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초록

This study aimed to confirm the induction of resistance to other drug classes by treating Mycobacterium abscessus with moxifloxacin, a fluoroquinolone used for treating nontuberculous mycobacteria infection, and to obtain genetic data for improving treatment. The reads were assembled and analyzed using reference strain sequence data, and the whole-genome and transcriptome sequences of four strains (MD2, MD4, MD6, and MD8) were reported. Antibiotic resistance was not induced by moxifloxacin treatment; however, transcriptomic analysis revealed that the expression of genes responding to stress was upregulated.

키워드

과제정보

This research was supported by the "Korea National Institute of Health" research project No. 2021-NI-014-01 and 2022-NI-013-01.

참고문헌

  1. Cowman S, van Ingen J, Griffith DE, Loebinger MR. 2019. Nontuberculous mycobacterial pulmonary disease. Eur. Respir. J. 54: 1900250.
  2. Koh W-J. 2017. Nontuberculous mycobacteria-overview. Microbiol. Spectr. 5: 10.1128/microbiolspec. tnmi1127-0024-2016.
  3. Daley CL, Iaccarino JM, Lange C, Cambau E, Wallace Jr RJ, Andrejak C, et al. 2020. Treatment of nontuberculous mycobacterial pulmonary disease: an official ATS/ERS/ESCMID/IDSA clinical practice guideline. Clin. Infect. Dis. 71: e1-e36. https://doi.org/10.1093/cid/ciaa241
  4. Kim J-Y, Kwak N, Yim J-J. 2022. The rise in prevalence and related costs of nontuberculous mycobacterial diseases in South Korea, 2010-2021. Open Forum Infecti. Dis. 9: ofac649.
  5. Yoon HJ, Choi HY, Ki M. 2017. Nontuberculosis mycobacterial infections at a specialized tuberculosis treatment centre in the Republic of Korea. BMC Infect. Dis. 17: 432.
  6. Gupta RS, Lo B, Son J. 2018. Phylogenomics and comparative genomic studies robustly support division of the genus Mycobacterium into an emended genus Mycobacterium and four novel genera. Front. Microbiol. 9: 67.
  7. Woods GL, Brown-Elliott BA, Conville PS, Desmond EP, Hall GS, Lin G, et al. 2019. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes. 2nd ed. Wayne (PA): Clinical and Laboratory Standards Institute; 2011 Mar. Report No.: M24-A2. CLSI Standards: Guidelines for Health Care Excellence.
  8. Guillemin I, Jarlier V, Cambau E. 1998. Correlation between quinolone susceptibility patterns and sequences in the A and B subunits of DNA gyrase in mycobacteria. Antimicrob. Agents Chemother. 42: 2084-2088. https://doi.org/10.1128/AAC.42.8.2084