Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Identification of a key signaling network regulating perennating bud dormancy in Panax ginseng

  • Jeoungeui Hong (Department of Biology, Chungbuk National University) ;
  • Soeun Han (Department of Biology, Chungbuk National University) ;
  • Kyoung Rok Geem (Department of Biology, Chungbuk National University) ;
  • Wonsil Bae (Department of Biology, Chungbuk National University) ;
  • Jiyong Kim (School of Biological Sciences, Seoul National University) ;
  • Moo-Geun Jee (Ginseng & Medicinal Plant Research Institute, Chungnam Agricultural Research & Extension Service) ;
  • Jung-Woo Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jang-Uk Kim (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Gisuk Lee (Department of Biological Sciences, Korea Advanced Institute for Science and Technology) ;
  • Youngsung Joo (School of Biological Sciences, Seoul National University) ;
  • Donghwan Shim (Department of Biological Sciences, Chungnam National University) ;
  • Hojin Ryu (Department of Biology, Chungbuk National University)
  • Received : 2024.02.14
  • Accepted : 2024.04.19
  • Published : 2024.09.01
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Abstract

Background: The cycle of seasonal dormancy of perennating buds is an essential adaptation of perennial plants to unfavorable winter conditions. Plant hormones are key regulators of this critical biological process, which is intricately connected with diverse internal and external factors. Recently, global warming has increased the frequency of aberrant temperature events that negatively affect the dormancy cycle of perennials. Although many studies have been conducted on the perennating organs of Panax ginseng, the molecular aspects of bud dormancy in this species remain largely unknown. Methods: In this study, the molecular physiological responses of three P. ginseng cultivars with different dormancy break phenotypes in the spring were dissected using comparative genome-wide RNA-seq and network analyses. These analyses identified a key role for abscisic acid (ABA) activity in the regulation of bud dormancy. Gene set enrichment analysis revealed that a transcriptional network comprising stress-related hormone responses made a major contribution to the maintenance of dormancy. Results: Increased expression levels of cold response and photosynthesis-related genes were associated with the transition from dormancy to active growth in perennating buds. Finally, the expression patterns of genes encoding ABA transporters, receptors (PYRs/PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs), and DELLAs were highly correlated with different dormancy states in three P. ginseng cultivars. Conclusion: This study provides evidence that ABA and stress signaling outputs are intricately connected with a key signaling network to regulate bud dormancy under seasonal conditions in the perennial plant P. ginseng.

Keywords

Acknowledgement

This work was supported by the Cooperative Research Program for Agriculture Science & Technology Development (No. PJ01482004).

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