Journal of Microbiology and Biotechnology

  • 제34권8호
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  • Pages.1627-1635
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Antagonistic Efficacy of Symbiotic Bacterium Xenorhabdus sp. SCG against Meloidogyne spp.

  • Jong-Hoon Kim (Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Byeong-Min Lee (Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hyung Chul Lee (ECOWIN Co., Ltd.) ;
  • In-Soo Choi (Nematode Research Center, Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kyung-Bon Koo (ECOWIN Co., Ltd.) ;
  • Kwang-Hee Son (Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2024.04.01
  • 심사 : 2024.07.01
  • 발행 : 2024.08.28
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초록

The inhabitation and parasitism of root-knot nematodes (RKNs) can be difficult to control, as its symptoms can be easily confused with other plant diseases; hence, identifying and controlling the occurrence of RKNs in plants remains an ongoing challenge. Moreover, there are only a few biological agents for controlling these harmful nematodes. In this study, Xenorhabdus sp. SCG isolated from entomopathogenic nematodes of genus Steinernema was evaluated for nematicidal effects under in vitro and greenhouse conditions. The cell-free filtrates of strain SCG showed nematicidal activity against Meloidogyne species J2s, with mortalities of > 88% at a final concentration of 10%, as well as significant nematicidal activity against the three other genera of plant-parasitic nematodes in a dose-dependent manner. Thymine was isolated as active compounds by assay-guided fractionation and showed high nematicidal activity against M. incognita. Greenhouse experiments suggested that cell-free filtrates of strain SCG efficiently controlled the nematode population in M. incognita-infested tomatoes (Solanum lycopersicum L., cv. Rutgers). In addition, a significant increase in host plant growth was observed after 45 days of treatment. To our knowledge, this is the first to demonstrate the nematicidal activity spectrum of isolated Xenorhabdus species and their application to S. lycopersicum L., cv. Rutgers under greenhouse conditions. Xenorhabdus sp. SCG could be a promising biological nematicidal agent with plant growth-enhancing properties.

키워드

과제정보

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through "Crop Viruses and Pests Response Industry Technology Development" Program (No. 321110-4) funded by Ministry of Agriculture, Food and Rural Af-fairs (MAFRA).

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