Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Internal and External Changes in Reactive Oxygen Species (ROS) During the Growth Stages of Harmful Algal Bloom Species

적조생물의 성장단계에 따른 세포 내·외 Reactive Oxygen Species (ROS) 변화

  • Minji Lee (South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Danbi Bang (South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Seong-Su Shin (South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Yoonja Kang (Department of Environmental Oceanography, Chonnam National University)
  • 이민지 (국립수산과학원 기후환경자원과) ;
  • 방단비 (국립수산과학원 기후환경자원과) ;
  • 신성수 (국립수산과학원 기후환경자원과) ;
  • 강윤자 (전남대학교 환경해양학과)
  • Received : 2024.05.31
  • Accepted : 2024.08.25
  • Published : 2024.08.31
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Abstract

Reactive oxygen species (ROS) generated by harmful algal blooms (HABs) exert detrimental effects on aquaculture systems. Fish gill cells deteriorate upon exposure to HABs, suggesting that internally generated ROS in HABs influences the external environment. Therefore, we investigated the internal and external changes in ROS concentrations during growth using fluorescence staining of four representative HABs: Alexandrium affine, Chattonella marina, Karenia mikimotoi, and Margalefidinium polykrikoides. The concentrations of H2O2 and O2- produced by A. affine were low; H2O2 from M. polykrikoides was primarily detected internally throughout the experiments, and O2- was not detected. High H2O2 and O2- concentrations were observed in K. mikimotoi during the death phase, with weak external O2- concentrations. Regarding C. marina, which produces large amounts of ROS, H2O2 was observed internally during the exponential phase, whereas weak O2- concentrations were measured externally in the stationary phases. Collectively, our results highlight that ROS concentrations and internal/external distributions are functions of HABs and growth stage. These differences indicate the potential allelopathic mechanisms of proliferating HABs and suggest a possible impact of ROS on aquaculture organisms.

Keywords

Acknowledgement

이 연구는 2024년 국립수산과학원 수산과학연구사업 R2024010과 정부 (과학기술정보통신부)의 재원으로 한국연구재단의 지원(NRF2022R1C1C1008380)을 받아 수행된 연구입니다.

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