Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Evaluation of the optimal thawing conditions for dog spermatozoa frozen in cryovials

  • Saddah Ibrahim (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine, Jeonbuk National University) ;
  • Yubyeol Jeon (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine, Jeonbuk National University) ;
  • Il-Jeoung Yu (Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine, Jeonbuk National University)
  • Received : 2024.04.09
  • Accepted : 2024.05.16
  • Published : 2024.06.30
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Abstract

Background: Using cryovial for freezing dog spermatozoa provides a practical method to increase extended sperm volume and shorten the time required for equilibration by using a simple freezing techniques. The purpose of this study was to determine the optimal thawing condition for dog sperm cryopreservation using cryovials. Methods: For sperm freezing, cryovials with 200 × 106 sperm/mL were cooled after the addition of tris egg yolk extender (TEY) at 4℃ for 20 min, then TEY with 4% glycerol was added and equilibrated for another 20 min before being aligned over LN2 vapor for another 20 min and plunged directly into LN2. Spermatozoa were thawed in a water bath at 37℃ for varying times (25 sec, 60 sec, 90 sec, and 120 sec) in the first experiment. In the second experiment, spermatozoa were thawed in a water bath at various temperatures and times (37℃ for 1 min, 37℃ for 1 min with gentle stirring, 24℃ for 24 min, and 75℃ for 20 sec). In these experiments, the effect of thawing conditions on motility parameters, viability (SYBR-14/PI), and acrosome integrity (PSA/FITC) of spermatozoa were investigated. Results: The post-thaw sperm motility parameters, viability, and acrosome integrity were not significantly different across the experimental groups. Conclusions: In this study, the characteristics of spermatozoa frozen using cryovials were not significantly affected by various thawing conditions.

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References

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