The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Anthracnose Caused by Colletotrichum nymphaeae on Strawberry Fruits in Korea

  • Je Hyeok Yoo (Strawberry Research Institute, Chungnam ARES) ;
  • Myung Soo Park (Department of Crops and Forestry, Korea National University of Agriculture and Fisheries) ;
  • Doo Hee Yi (Strawberry Research Institute, Chungnam ARES) ;
  • Myeong Hyeon Nam (Industrial Entomology Research Institute, Chungnam ARES)
  • Received : 2024.05.14
  • Accepted : 2024.06.10
  • Published : 2024.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Anthracnose fruit rot (AFR) has been observed in greenhouses during the harvesting period in the Chungnam Province of South Korea. Fruits infected inside the greenhouse show black or brown spots, orange conidial masses and in some areas of the infected parts, white fungal growths are visible. The size of these spots gradually expands, leading to the necrosis of the fruits and flowers. Three isolates were obtained from infected fruits and identified as strains of the Colletotrichum acutatum species complex based on morphological characteristics. Multilocus sequence analysis of actin, chitin synthase, glyceraldehyde-3-phosphate dehydrogenase genes, and internal transcribed spacer rDNA regions revealed that the isolates belong to a monophyletic group with the type strain of C. nymphaeae. This is the first time C. nymphaeae has been confirmed in strawberry fruit in Korea.

Keywords

Acknowledgement

This study was funded by a grant under the Rural Development Administration (Project No. PJ01533202).

References

  1. Korea Statistical Information Service. Cultivated area of fruit and vegetable crops and Income of greenhouse crops [Internet]. Daejeon: Statistics Korea; 2024 [cited 2024 May 20]. Available from: https://kosis.kr.
  2. Kim TI, Jang WS, Nam MH, Lee WK, Lee SS. Breeding of strawberry Seolhyang for forcing culture. IHC 2006;231.
  3. Kim HS, Jang WS, Kim TI, Nam MH, Lee IH, Lee HC. Breeding of big size strawberry 'Kingsberry' for forcing culture. Kor J Hort Sci Technol 2016;34:167.
  4. Nam MH, Kim TI, Gleason ML, Song JY, Kim HG. First report on anthracnose fruit rot caused by Colletotrichum acutatum on strawberry in Korea. Plant Dis 2008;92:1247.
  5. Nam MH, Yoo JH, Yun TS, Kim HH, Kim HG. Anthracnose of strawberry: Etiological and ecological characteristics and management. Res Plant Dis 2023;29:205-19. https://doi.org/10.5423/RPD.2023.29.3.205
  6. Nam MH, Park MS, Lee HD, Yu SH. Taxonomic re-evaluation of Colletotrichum gloeosporioides isolated from strawberry in Korea. Plant Pathol J 2013;29:317-22. https://doi.org/10.5423/PPJ.NT.12.2012.0188
  7. Prihastuti H, Cai L, Chen H, McKenzie EHC, Hyde KD. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Divers 2009;39:89-109.
  8. Damm U, Cannon PF, Woudenberg JHC, Crous PW. The Colletotrichum acutatum species complex. Stud Mycol 2012;73:37-113. https://doi.org/10.3114/sim0010
  9. Zhang L, Song L, Xu X, Zou X, Duan K, Gao Q. Characterization and fungicide sensitivity of Colletotrichum species causing strawberry anthracnose in eastern China. Plant Dis 2020;104:1960-8. https://doi.org/10.1094/PDIS-10-19-2241-RE
  10. Rogers SO, Bendich AJ. Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin SB, Schilperoort RA, editors. Plant molecular biology manual. Dordrecht: Springer Netherlands; 1994. p. 183-90.
  11. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Bio Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  12. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Mol Biol Evol 2013;30:772-80. https://doi.org/10.1093/molbev/mst010
  13. Stamatakis A. RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006;22:2688-90. https://doi.org/10.1093/bioinformatics/btl446
  14. Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES science gateway for inference of large phylogenetic trees. LA: SC10 workshop on gateway computing environments (GCE10); 2010. pp. 1-8.
  15. Abo-Elwafa TM, Ragab SSM, Nehela Y, Essa TA. First report of strawberry anthracnose caused by Colletotrichum nymphaeae in Egypt. New Dis Rep 2023;48:e12205.
  16. Fernandez LM, Maumary RL, Seimandi GM, Pernuzzi C, Derita MG, Favaro MA. First report of anthracnose caused by Colletotrichum nymphaeae on strawberry fruits in central Argentina. Plant Dis 2024;108:221.
  17. Kim GH, Choi DH, Park SY, Koh YJ. First report of anthracnose caused by Colletotrichum nymphaeae on kiwiberry in Korea. Plant Dis 2018;102:1455.
  18. Chang TH, Hassan O, Lee YS. First report of anthracnose of Japanese plum (Prunus salicina) caused by Colletotrichum nymphaeae in Korea. Plant Dis 2018;102:1461.
  19. Hassan O, Lee DW, Chang T. First report of anthracnose of persimmon caused by Colletotrichum nymphaeae in Korea. Plant Dis 2019;103:1772.