초록
본 연구에서는 전나무와 잣나무 잎으로부터 항균특성을 갖는 기능성 물질을 추출한 후 반응표면분석법 중 중심합성 계획법(CCD-RSM)을 이용하여 최적화하였다. 최적 추출 조건은 전나무의 경우 추출온도(59.5 ℃)와 주정/초순수 부피비(69.5 vol.%)이었으며, 이때 반응치는 추출수율(13.5%), 대장균 억제직경(11.6 mm), 황색포도상구균 억제직경(9.3 mm)로 산출되었다. 또한 잣나무의 경우에는 최적 추출조건인 추출온도(59.2 ℃)와 주정/초순수 부피비(67.8 vol.%)에 서 추출수율(4.8%), 대장균 억제직경(7.9 mm), 황색포도상구균 억제직경(12.5 mm)로 산출되었다. 각 천연물의 최적조건에서 실제 실험 결과 전나무 잎의 추출수율은 13.0%, 대장균 억제직경은 11.7 mm, 황색포도상구균 억제직경은 9.2 mm로 약 ± 2.30% 오차율을 나타내며, 잣나무 잎의 추출수율은 5.1%, 대장균 억제직경은 7.5 mm, 황색포도상구균 억제직경은 12.3 mm로 ± 4.23% 오차율을 나타내었다.
In this study, functional substances with antibacterial properties were extracted from the needles of Abies holophylla and Pinus koraiensis, and optimized using the central composite design-response surface methodology (CCD-RSM). The optimal extraction conditions for Abies holophylla were an extraction temperature of 59.5 ℃ and an ethanol/ultrapure water volume ratio of 69.5 vol.%, resulting in an extraction yield of 13.5% and inhibition diameters of 11.6 mm against Escherichia coli (E. coli) and 9.3 mm against Staphylococcus aureus (S. aureus). For Pinus koraiensis, the optimal extraction conditions were an extraction temperature of 59.2 ℃ and an ethanol/ultrapure water volume ratio of 67.8 vol.%, resulting in an extraction yield of 4.8% and inhibition diameters of 7.9 mm against E. coli and 12.5 mm against S. aureus. The actual experimental results under these optimal conditions showed that an extraction yield from Abies holophylla needles was 13.0% and an inhibition diameter of 11.7 mm against E. coli and 9.2 mm against S. aureus, indicating an error rate of approximately ± 2.3%. For Pinus koraiensis needles, the extraction yield was 5.1%, with inhibition diameters of 7.5 mm against E. coli and 12.3 mm against S. aureus, indicating an error rate of ± 4.23%.