Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Anti-inflammatory Mechanism of Blueberry is through Suppression of NF-kB/Caspase-1 Activation in LPS-induced RAW264.7 Cells

  • Mi-Ok Yang (Wonkwang Institute of Nature Healthcare Studies) ;
  • Noh-Yil Myung (Department of Korean Medicine and Food Therapy, Wonkwang Digital University)
  • Received : 2024.04.22
  • Accepted : 2024.05.16
  • Published : 2024.06.01
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Abstract

Blueberry (BB), fruit of Vacciniumi, has been hailed as an antioxidant superfood. BB is a rich source of vitamins, minerals, flavonoids, phenolic acids and known to have a variety of pharmacological actions. The purpose of this work is to clarify the anti-inflammatory mechanism of BB in lipopolysaccharide (LPS)-activated RAW264.7 macrophage. We explored the effects of BB on the production of inflammatory cytokines, prostaglandin E2 (PGE2) and expression of cyclooxygenase (COX)-2 in LPS-activated RAW264.7 macrophage. Moreover, to investigate the molecular mechanisms by BB, we evaluated whether BB modulate nuclear factor-kappa B (NF)-kB pathway and caspase- 1 activation. The findings of this work demonstrated that BB alleviated the LPS-enhanced inflammatory cytokines and PGE2, as well as COX-2 levels. Additionally, we demonstrated that the anti-inflammatory mechanism of BB occurs due to the attenuation of IκB-α degradation, NF-kB translocation and caspase-1 activation. Conclusively, these findings provide evidence that BB may be useful agents in the treatment of inflammation.

Keywords

Acknowledgement

This study was supported by Wonkwang Digital University in 2024.

References

  1. Broz, P., J. von Moltke, J.W. Jones, R.E. Vance and D.M. Monack. 2010. Differential requirement for Caspase-1 autoproteolysis in pathogen-induced cell death and cytokine processing. Cell Host Microbe. 8:471-483. https://doi.org/10.1016/j.chom.2010.11.007
  2. Celec, P. 2004. Nuclear factor kappa B-molecular biomedicine: the next generation. Biomed. Pharmacother. 58:365-371. https://doi.org/10.1016/j.biopha.2003.12.015
  3. Chen, C.F., Y.D. Li and Z. Xu. 2010. Chemical principles and bioactivities of blueberry. Yao Xue Xue Bao. 45:422-429.
  4. Christiaens, I., D.B. Zaragoza, L. Guilbert, S.A. Robertson, B.F. Mitchell and D.M. Olson. 2008. Inflammatory processes in preterm and term parturition. J. Reprod. Immunol. 79:50-57. https://doi.org/10.1016/j.jri.2008.04.002
  5. Du, C., M. Bhatia, S.C. Tang, M. Zhang and T. Steiner. 2015. Mediators of inflammation: Inflammation in cancer, chronic diseases, and wound healing. Mediators. Inflamm. 2015:570653.
  6. Fiorucci, S., L. Santucci, J.L. Wallace, M. Sardina, M. Romano, P. del Soldato and A. Morelli. 2003. Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa. Proc. Natl. Acad. Sci. 100:10937-10941. https://doi.org/10.1073/pnas.1933204100
  7. Fortunato, S.J., R. Menon and S.J. Lombardi. 2002. Role of tumor necrosis factor-alpha in the premature rupture of membranes and preterm labor pathways. Am. J. Obstet. Gynecol. 187:1159-1162. https://doi.org/10.1067/mob.2002.127457
  8. Ghosh, S. and H.S. Hayden. 2008. New regulators of NF-κB in inflammation. Nat. Rev. Immunol. 8:837-848. https://doi.org/10.1038/nri2423
  9. Guan, M., H. Ma, X. Fan, X. Chen, M. Miao and H. Wu. 2020. Dexamethasone alleviate allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome. Int. Immunopharmacol. 78:106017.
  10. Han, N.R., P.D. Moon, N.R. Kim, H.Y. Kim, H.J. Jeong and H.M. Kim. 2017. Schisandra chinensis and its main constituent schizandrin attenuate allergic reactions by downregulating caspase-1 in ovalbumin-sensitized mice. Am. J. Chin. Med. 45:159-172. https://doi.org/10.1142/S0192415X17500112
  11. Harris, S.G., J. Padilla, L. Koumas, D. Ray and R.P. Phipps. 2002. Prostaglandins as modulators of immunity. Trends. Immunol. 23:144-150. https://doi.org/10.1016/S1471-4906(01)02154-8
  12. Jeon, Y.D., S.H. Lee and S.J. Kim. 2021. Undaria pinnatifida inhibits the mast cell-mediated inflammatory response via NF-κB/Caspase-1 suppression. Korean J. Plant Res. 34:503-509.
  13. Kausar, H., J. Jeyabalan, F. Aqil, D. Chabba, J. Sidana, I.P. Singh and R.C. Gupta. 2012. Berry anthocyanidins synergistically suppress growth and invasive potential of human non-small-cell lung cancer cells. Cancer Lett. 325:54-62. https://doi.org/10.1016/j.canlet.2012.05.029
  14. Kim, C.H., Y.K. Lee, M.J. Kim, J.S. Choi, B.S. Hwang, P.Y. Cho, Y.J. Kim and Y.T. Jeong. 2023. Evaluation antioxidant and anti-inflammatory activity of ethanolic extracts of Myriophyllum spicatum L. in lipopolysaccharide-stimulated RAW 264.7 cells. Korean J. Plant Res. 36:15-25.
  15. Liu, Y.C., X.B. Zou, Y.F. Chai and Y.M. Yao. 2014. Macrophage polarization in inflammatory diseases. Int. J. Biol. Sci. 10:520-529. https://doi.org/10.7150/ijbs.8879
  16. Majdalawieh, A. and H.S. Ro. 2010. Regulation of IκBα function and NF-κB signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators. Inflamm. 2010:823-821.
  17. Mann, J.R., M.G. Backlund and R.N. DuBois. 2005. Mechanisms of disease: Inflammatory mediators and cancer prevention. Nat. Clin. Pract. Oncol. 2:202-210. https://doi.org/10.1038/ncponc0140
  18. Martinez, F.O., A. Sica, A. Mantovani and M. Locati. 2008. Macrophage activation and polarization. Front. Biosci. 13:453-461. https://doi.org/10.2741/2692
  19. Minghetti, L. 2004. Cyclooxygenase-2 (COX-2) in inflammatory and degenerative brain diseases. J. Neuropathol. Exp. Neurol. 63:901-910. https://doi.org/10.1093/jnen/63.9.901
  20. Moon, P.D. and H.M. Kim. 2011. Thymic stromal lymphopoietin is expressed and produced by caspase-1/NF-κB pathway in mast cells. Cytokine 54:239-243. https://doi.org/10.1016/j.cyto.2011.03.007
  21. Skrovankova, S., D. Sumczynski, J. Mlcek, T. Jurikova and J. Sochor. 2015. Bioactive compounds and antioxidant activity in different types of berries. Int. J. Mol. Sci. 16:24673-24706. https://doi.org/10.3390/ijms161024673
  22. Wu, T., Q. Tang, Z. Gao, Z. Yu, H. Song, X. Zheng and W. Chen. 2013. Blueberry and mulberry juice prevent obesity development in C57BL/6 mice. PLoS ONE 8:e77585.
  23. Zhang, L. and C.C. Wang. 2014. Inflammatory response of macrophages in infection. Hepatob. Pancreatic. Dis. Int. 13:138-152. https://doi.org/10.1016/S1499-3872(14)60024-2