Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
권호 표지
DOI QR코드

DOI QR Code

Acoustofluidic Separation of Elastic and Rigid Microspheres

탄성 및 강성 마이크로입자의 음향미세유체역학적 분리

  • Mushtaq Ali (Department of Mechanical Engineering, Chonnam National University) ;
  • Song Ha Lee (Department of Mechanical Engineering, Chonnam National University) ;
  • Jinsoo Park (Department of Mechanical Engineering, Chonnam National University)
  • 무스타크 알리 ;
  • 이송하 ;
  • 박진수
  • Received : 2024.06.20
  • Accepted : 2024.07.05
  • Published : 2024.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

Microparticle separation has demonstrated significant potential for biological, chemical, and medical applications. We introduce a surface acoustic wave (SAW)-based microfluidic device for separation of elastic and rigid microspheres based on their property and size. By tuning the SAWs to match the resonant frequencies of certain microspheres, those particles could be selectively separated from the other microspheres. When microspheres are exposed to an acoustic field, they experience the SAW-induced acoustic radiation force (ARF), whose magnitude is dependent on the microparticle size and properties. We modeled the SAW-induced ARF based on elastic sphere theory and conducted a series of experiments to separate elastic and rigid microspheres. We further utilized the acoustofluidic method for the separation of Thalassiosira Eccentrica microalgae based on the differences in their sizes with purity exceeding 90%. We anticipate that our technique will open up new possibilities for sample preparation, detection, and diagnosis in various emerging biological and medical analyses.

Keywords

Acknowledgement

This work was supported by the Korea Industrial Complex Corporation (KICOX) Competitiveness reinforcement project for industrial clusters grants funded by the Korea government (MOTIE) (No. 1415189052). The microfluidic devices were fabricated by using a mask aligner (MDA-400S, MIDAS) at Energy Convergence Core Facility in Chonnam National University.

References

  1. Fraser, Andrew G., Ravi S. Kamath, Peder Zipperlen, Maruxa Martinez-Campos, Marc Sohrmann, and Julie Ahringer. "Functional genomic analysis of C. elegans chromosome I by systematic RNA interference." Nature 408, no. 6810 (2000): 325-330. 
  2. Wang, Zeyu, Haichen Wang, Ryan Becker, Joseph Rufo, Shujie Yang, Brian E. Mace, Mengxi Wu, Jun Zou, Daniel T. Laskowitz, and Tony Jun Huang. "Acoustofluidic separation enables early diagnosis of traumatic brain injury based on circulating exosomes." Microsystems & Nanoengineering 7, no. 1 (2021): 20. 
  3. Xie, Yuliang, Hunter Bachman, and Tony Jun Huang. "Acoustofluidic methods in cell analysis." TrAC Trends in Analytical Chemistry 117 (2019): 280-290. 
  4. Hejazian, Majid, Weihua Li, and Nam-Trung Nguyen. "Lab on a chip for continuous-flow magnetic cell separation." Lab on a Chip 15, no. 4 (2015): 959-970. 
  5. Akagi, Takanori, and Takanori Ichiki. "Cell electrophoresis on a chip: what can we know from the changes in electrophoretic mobility?." Analytical and bioanalytical chemistry 391 (2008): 2433-2441. 
  6. Julio, Raihan Hadi, Muhammad Soban Khan, Mushtaq Ali, Ghulam Destgeer, and Jinsoo Park. "Size-based Separation of Yeast Cell by Surface Acoustic Wave-induced Acoustic Radiation Force." Journal of the Korean Society of Visualization 21, no. 3 (2023): 93-100. 
  7. Huang, Lotien Richard, Edward C. Cox, Robert H. Austin, and James C. Sturm. "Continuous particle separation through deterministic lateral displacement." Science 304, no. 5673 (2004): 987-990. 
  8. MacDonald, Michael P., Gabriel C. Spalding, and Kishan Dholakia. "Microfluidic sorting in an optical lattice." Nature 426, no. 6965 (2003): 421-424. 
  9. Ha, Byung Hang, Kang Soo Lee, Ghulam Destgeer, Jinsoo Park, Jin Seung Choung, Jin Ho Jung, Jennifer Hyunjong Shin, and Hyung Jin Sung. "Acoustothermal heating of polydimethylsiloxane microfluidic system." Scientific reports 5, no. 1 (2015): 11851. 
  10. Bhagat, Ali Asgar S., Hansen Bow, Han Wei Hou, Swee Jin Tan, Jongyoon Han, and Chwee Teck Lim. "Microfluidics for cell separation." Medical & biological engineering & computing 48 (2010): 999-1014. 
  11. Ali, Mushtaq, and Jinsoo Park. "Ultrasonic surface acoustic wave-assisted separation of microscale droplets with varying acoustic impedance." Ultrasonics Sonochemistry 93 (2023): 106305. 
  12. Destgeer, Ghulam, Byung Hang Ha, Jinsoo Park, Jin Ho Jung, Anas Alazzam, and Hyung Jin Sung. "Travelling surface acoustic waves microfluidics." Physics Procedia 70 (2015): 34-37. 
  13. Amstad, Esther, Frans Spaepen, Michael P. Brenner, and David A. Weitz. "The microfluidic nebulator: production of sub-micrometer sized airborne drops." Lab on a Chip 17, no. 8 (2017): 1475-1480. 
  14. Ahmed, Husnain, Jinsoo Park, Ghulam Destgeer, Muhammad Afzal, and Hyung Jin Sung. "Surface acoustic wave-based micromixing enhancement using a single interdigital transducer." Applied Physics Letters 114, no. 4 (2019). 
  15. Khan, Muhammad Soban, Mushtaq Ali, Song Ha Lee, Keun Young Jang, Seong Jae Lee, and Jinsoo Park. "Acoustofluidic separation of prolate and spherical micro-objects." Microsystems & Nanoengineering 10, no. 1 (2024): 6. 
  16. Bourquin, Yannyk, Abeer Syed, Julien Reboud, Lisa C. Ranford-Cartwright, Michael P. Barrett, and Jonathan M. Cooper. "Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics." Angewandte Chemie International Edition 53, no. 22 (2014): 5587-5590. 
  17. Augustsson, Per, Jonas T. Karlsen, Hao-Wei Su, Henrik Bruus, and Joel Voldman. "Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping." Nature communications 7, no. 1 (2016): 11556. 
  18. Destgeer, Ghulam, Jin Ho Jung, Jinsoo Park, Husnain Ahmed, Kwangseok Park, Raheel Ahmad, and Hyung Jin Sung. "Acoustic impedance-based manipulation of elastic microspheres using travelling surface acoustic waves." RSC advances 7, no. 36 (2017): 22524-22530. 
  19. Ma, Zhichao, David J. Collins, Jinhong Guo, and Ye Ai. "Mechanical properties based particle separation via traveling surface acoustic wave." Analytical chemistry 88, no. 23 (2016): 11844-11851. 
  20. Ali, Mushtaq, Song Ha Lee, Beomseok Cha, Woohyuk Kim, Nomin-Erdene Oyunbaatar, Dong-Weon Lee, and Jinsoo Park. "Acoustofluidic separation of cell-encapsulated droplets based on traveling surface acoustic wave-induced acoustic radiation force." Sensors and Actuators B: Chemical (2024): 135988. 
  21. Sahin, Mehmet A., Mushtaq Ali, Jinsoo Park, and Ghulam Destgeer. "Fundamentals of Acoustic Wave Generation and Propagation." Acoustic Technologies in Biology and Medicine (2023): 1-36. 
  22. Hasegawa, Takahi, and Katuya Yosioka. "Acoustic-radiation force on a solid elastic sphere." The Journal of the Acoustical Society of America 46, no. 5B (1969): 1139-1143. 
  23. Brandrup, J., Immergut, E.H., Grulke, E.A., Abe, A. and Bloch, D.R. eds., 1999. Polymer handbook (Vol. 89, pp. V87-V90). New York: Wiley. 
  24. Hopkins, C.C., Haward, S.J. and Shen, A.Q., 2020. Purely elastic fluid-structure interactions in microfluidics: implications for mucociliary flows. Small, 16(9), p.1903872.