Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Correlation between Rab3A Expression and Sperm Kinematic Characteristics

  • Seung-Ik Jang (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jae-Hwan Jo (Department of Animal Biotechnology, Kyungpook National University) ;
  • Uwamahoro Claudine (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Eun-Ju Jung (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Woo-Jin Lee (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Ju-Mi Hwang (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jeong-Won Bae (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Dae-Hyun Kim (Department of Animal Science, Chonnam National University) ;
  • Jun Koo Yi (School of Animal Life Convergence Science, Hankyong National University) ;
  • Jae Jung Ha (Gyeongbuk Livestock Research Institute) ;
  • Dong Yep Oh (Gyeongbuk Livestock Research Institute) ;
  • Woo-Sung Kwon (Department of Animal Science and Biotechnology, Kyungpook National University)
  • Received : 2024.01.04
  • Accepted : 2024.02.22
  • Published : 2024.03.31
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Abstract

Ras-related (Rab) proteins, integral members of the monomeric G-protein family, play a pivotal role in regulating intracellular vesicular transport. These proteins contribute to male reproductive processes, specifically in acrosome formation, exocytosis, and sperm motility. Although a prior study indicated a correlation between Rab3A and sperm motility, including motion kinematic parameters such as mean dance, this association has only been explored within a limited sample size. Therefore, further verification is required to confirm the correlation between Rab3A and sperm motility parameters. In the present study, Rab3A expression, sperm motility, and motion kinematic parameters were analyzed in 150 boar spermatozoa. Additionally, correlations between Rab3A expression and sperm kinematic characteristics were evaluated statistically. The results revealed significant associations between Rab3A protein expression levels and various motion kinematic parameters. Specifically, Rab3A levels exhibited positive correlations with average path velocity (p<0.05), mean amplitude of lateral head displacement (p<0.05), and curvilinear velocity (p<0.01). Consequently, it is proposed that Rab3A protein plays a crucial role in male fertility through its correlation with sperm kinematic characteristics, making it a potential marker for sperm motility-related assessments.

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References

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