BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Prmt7 is required for the osteogenic differentiation of mesenchymal stem cells via modulation of BMP signaling

  • Tuan Anh Vuong (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • Yan Zhang (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • June Kim (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • Young-Eun Leem (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • Jong-Sun Kang (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
  • 투고 : 2023.10.26
  • 심사 : 2024.01.15
  • 발행 : 2024.07.31
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초록

Arginine methylation, which is catalyzed by protein arginine methyltransferases (Prmts), is known to play a key role in various biological processes. However, the function of Prmts in osteogenic differentiation of mesenchymal stem cells (MSCs) has not been clearly understood. In the current study, we attempted to elucidate a positive role of Prmt7 in osteogenic differentiation. Prmt7-depleted C3H/10T1/2 cells or bone marrow mesenchymal stem cells (BMSCs) showed the attenuated expression of osteogenic specific genes and Alizarin red staining compared to the wild-type cells. Furthermore, we found that Prmt7 deficiency reduced the activation of bone morphogenetic protein (BMP) signaling cascade, which is essential for the regulation of cell fate commitment and osteogenesis. Taken together, our data indicate that Prmt7 plays important regulatory roles in osteogenic differentiation.

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과제정보

This research was supported by the National Research Foundation of Kore (NRF) Grant funded by the Korean Government (MSIP) (NRF-2022R1A2B5B02001482 to JSK) and (NRF-2018R1D1A1B07041661; NRF-2021R1I1A1A01050302 to YEL).

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