Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant activity and neuroprotective effect of ethanolic extract of Polygonum multiflorum

적하수오(Polygonum multiflorum) 에탄올 추출물의 항산화 활성 및 뇌 신경세포 보호효과

  • Hye Ji Choi (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Hyo Lim Lee (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Min Ji Go (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ju Hui Kim (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Han Su Lee (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • In Young Kim (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ho Jin Heo (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 최혜지 (경상국립대학교 응용생명과학부(BK21)) ;
  • 이효림 (경상국립대학교 응용생명과학부(BK21)) ;
  • 고민지 (경상국립대학교 응용생명과학부(BK21)) ;
  • 김주희 (경상국립대학교 응용생명과학부(BK21)) ;
  • 이한수 (경상국립대학교 응용생명과학부(BK21)) ;
  • 김인영 (경상국립대학교 응용생명과학부(BK21)) ;
  • 허호진 (경상국립대학교 응용생명과학부(BK21))
  • Received : 2024.04.15
  • Accepted : 2024.05.17
  • Published : 2024.06.30
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Abstract

This study evaluated the in vitro antioxidant activities of ethanolic Polygonum multiflorum (P. multiflorum) extracts and their cytoprotective effects on H2O2-induced HT22 and SK-N-MC cells. Among ethanolic extracts of P. multiflorum, the 40% ethanolic extract of P. multiflorum exhibited high total phenolics and flavonoid contents, with 105.68 mg of GAE/g and 28.92 mg of RE/g, respectively. The 40% ethanolic extract of P. multiflorum showed a high 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and malondialdehyde (MDA) inhibitory effect. The 40% ethanolic extract of P. multiflorum also showed efficient inhibitory activity against α-glucosidase and acetylcholinesterase. Moreover, the 40% ethanolic extract of P. multiflorum reduced oxidative stress and increased cell viability in H2O2-induced HT22 and SK-N-MC cells as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) and 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. High-performance liquid chromatography (HPLC) identified 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (TSG) as the bioactive compound in the 40% ethanolic extract of P. multiflorum.

본 연구를 통해 적하수오(Polygonum multiflorum) 에탄올 추출물이 갖는 in vitro 항산화 활성 및 뇌 신경세포 보호 효과를 확인하고자 하였다. 적하수오 0%, 20%, 40%, 60%, 80% 및 95% 에탄올 추출물 중 40% 에탄올 추출물이 다른 추출물에 비해 상대적으로 많은 총페놀성 화합물 및 총플라보노이드 함량을 나타냈으며, 적하수오 40% 에탄올 추출물은 우수한 ABTS 라디칼 소거 활성과 MDA 생성 억제 효과를 나타내었다. 특히, 40% 에탄올 추출물은 높은 α-glucosidase 및 acetylcholinesterase (AChE) 억제 활성을 나타내었다. 또한, 적하수오 40% 에탄올 추출물은 H2O2 유도 HT22와 SK-N-MC 세포에서 세포 생존율을 증가시키고 세포 내 산화적 스트레스를 감소시킴으로써 효과적인 세포 보호 효과를 나타냈다. 마지막으로, HPLC 분석결과 적하수오 40% 에탄올 추출물의 주요 생리활성 물질은 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside(TSG)로 확인되었다. 따라서, 본 연구에서 나타난 적하수오의 항산화 활성 및 뇌 신경세포 보호 효과는 TSG를 포함한 다양한 생리활성물질에서 기인한 것으로 판단되며, 건강기능식품에 소재로서의 활용 가능성이 기대된다.

Keywords

Acknowledgement

This study is the result of research conducted with support from the Brain Korea (BK) 21 program. Thank you for this.

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