Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery

  • Hye Seon Song (Division of Environmental Materials, Honam National Institute of Biological Resource (HNIBR)) ;
  • Yeon Bee Kim (Kimchi Functionality Research Group, World Institute of Kimchi) ;
  • Joon Yong Kim (Microbiome Research Institute, LISCure Biosciences Inc.) ;
  • Seong Woon Roh (Microbiome Research Institute, LISCure Biosciences Inc.) ;
  • Tae Woong Whon (Kimchi Functionality Research Group, World Institute of Kimchi)
  • Received : 2023.11.17
  • Accepted : 2024.02.03
  • Published : 2024.04.28
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Abstract

Despite considerable advancements achieved using next-generation sequencing technologies in exploring microbial diversity, several species of the gut microbiome remain unknown. In this transformative era, culturomics has risen to prominence as a pivotal approach in unveiling realms of microbial diversity that were previously deemed inaccessible. Utilizing innovative strategies to optimize growth and culture medium composition, scientists have successfully cultured hard-tocultivate microbes. This progress has fostered the discovery and understanding of elusive microbial entities, highlighting their essential role in human health and disease paradigms. In this review, we emphasize the importance of culturomics research on the gut microbiome and provide new theories and insights for expanding microbial diversity via the optimization of cultivation conditions.

Keywords

Acknowledgement

This research was supported by the World Institute of Kimchi (KE2401-1-1), the Main Research Program of the Korea Food Research Institute (E0170600-07) funded by the Ministry of Science and ICT, a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2022R1C1C1003195), and a grant from the Honam National Institute of Biological Resources (HNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (HNIBR202302117).

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