Journal of Microbiology and Biotechnology

  • 제34권3호
  • /
  • Pages.538-546
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Transcriptional Response of Pectobacterium carotovorum to Cinnamaldehyde Treatment

  • Jihye Jung (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Dawon Jo (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Soo-Jin Kim (Division of Agricultural Microbiology, National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2023.11.27
  • 심사 : 2023.12.21
  • 발행 : 2024.03.28
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초록

Cinnamaldehyde is a natural compound extracted from cinnamon bark essential oil, acclaimed for its versatile properties in both pharmaceutical and agricultural fields, including antimicrobial, antioxidant, and anticancer activities. Although potential of cinnamaldehyde against plant pathogenic bacteria like Agrobacterium tumefaciens and Pseudomonas syringae pv. actinidiae causative agents of crown gall and bacterial canker diseases, respectively has been documented, in-depth studies into cinnamaldehyde's broader influence on plant pathogenic bacteria are relatively unexplored. Particularly, Pectobacterium spp., gram-negative soil-borne pathogens, notoriously cause soft rot damage across a spectrum of plant families, emphasizing the urgency for effective treatments. Our investigation established that the Minimum Inhibitory Concentrations (MICs) of cinnamaldehyde against strains P. odoriferum JK2, P. carotovorum BP201601, and P. versatile MYP201603 were 250 ㎍/ml, 125 ㎍/ml, and 125 ㎍/ml, respectively. Concurrently, their Minimum Bactericidal Concentrations (MBCs) were found to be 500 ㎍/ml, 250 ㎍/ml, and 500 ㎍/ml, respectively. Using RNA-sequencing analysis, we identified 1,907 differentially expressed genes in P. carotovorum BP201601 treated with 500 ㎍/ml cinnamaldehyde. Notably, our results indicate that cinnamaldehyde upregulated nitrate reductase pathways while downregulating the citrate cycle, suggesting a potential disruption in the aerobic respiration system of P. carotovorum during cinnamaldehyde exposure. This study serves as a pioneering exploration of the transcriptional response of P. carotovorum to cinnamaldehyde, providing insights into the bactericidal mechanisms employed by cinnamaldehyde against this bacterium.

키워드

과제정보

This research was supported by grants from the Agenda Project (Agenda Project Nos. PJ01679901) of the Rural Development Administration (RDA), South Korea.

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