Journal of Microbiology and Biotechnology

  • 제34권2호
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  • Pages.476-483
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Immune Enhancement Effects of Neutral Lipids, Glycolipids, Phospholipids from Halocynthia aurantium Tunic on RAW264.7 Macrophages

  • A-yeong Jang (Department of Wellness-Bio Industry, Gangneung-Wonju National University) ;
  • Weerawan Rod-in (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • Il-shik Shin (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • Woo Jung Park (Department of Wellness-Bio Industry, Gangneung-Wonju National University)
  • 투고 : 2023.07.05
  • 심사 : 2023.10.30
  • 발행 : 2024.02.28
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초록

Fractionated lipids of Halocynthia aurantium (Pyuridae) have been demonstrated to possess anti-inflammatory properties. However, their modulatory properties have not been reported yet. Thus, the objective of this study was to determine immune enhancing effects of fractionated lipids from H. aurantium tunic on macrophage cells. The tunic of H. aurantium was used to isolate total lipids, which were then subsequently separated into neutral lipids, glycolipids, and phospholipids. RAW264.7 cells were stimulated with different concentrations (0.5, 1.0, 2.0, and 4.0%) of each fractionated lipid. Cytotoxicity, production of NO, expression levels of immune-associated genes, and signaling pathways were then determined. Neutral lipids and glycolipids significantly stimulated NO and PGE2 production and expression levels of IL-1β, IL-6, TNF-α, and COX-2 in a dose-dependent manner, while phospholipids ineffectively induced NO production and mRNA expression. Furthermore, it was found that both neutral lipids and glycolipids increased NF-κB p-65, p38, ERK1/2, and JNK phosphorylation, suggesting that these lipids might enhance immunity by activating NF-κB and MAPK signaling pathways. In addition, H. aurantium lipids-induced TNF-α expression was decreased by blocking MAPK or NF-κB signaling pathways. Phagocytic activity of RAW 264.7 cells was also significantly enhanced by neutral lipids and glycolipids. These results suggest that neutral lipids and glycolipids from H. aurantium tunic have potential as immune-enhancing materials.

키워드

과제정보

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (RS-2023-00248832). This research project was also supported by the University Emphasis Research Institute Support Program (No.2018R1A61A03023584), which is funded by National Research Foundation of Korea. Additionally, this research were supported by the Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (20220042, Korea Sea Grant Program: Gangwon Sea Grant) and supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the "Regional Specialized lndustry Development Pius Prograrn (R&D, S3258709)" supervised by the Korea Technology and Information Promotion Agency for SMEs (TIPA).

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