Journal of Gastric Cancer

  • 제24권3호
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  • Pages.246-256
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  • 2024
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  • 2093-582X(pISSN)
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  • 2093-5641(eISSN)
대한위암학회 (The Korean Gastric Cancer Association)
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Efficacy of Hyperthermic Pressurized Intraperitoneal Aerosol Chemotherapy in an In Vitro Model Using a Human Gastric Cancer AGS Cell Line and an Abdominal Cavity Model

  • Sa-Hong Min (Department of Gastrointestinal Surgery, Asan Medical Center) ;
  • Jieun Lee (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Mira Yoo (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Duyeong Hwang (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Eunju Lee (Department of Surgery, Chung-Ang University Gwangmyeong Hospital) ;
  • So Hyun Kang (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Kanghaeng Lee (Department of Surgery, St. Vincent's Hospital) ;
  • Young Suk Park (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Sang-Hoon Ahn (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Yun-Suhk Suh (Department of Surgery, Seoul National University Bundang Hospital) ;
  • Do Joong Park (Department of Surgery, Seoul National University Hospital) ;
  • Hyung-Ho Kim (Department of Surgery, Seoul National University Bundang Hospital)
  • 투고 : 2024.03.15
  • 심사 : 2024.04.01
  • 발행 : 2024.07.01
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초록

Purpose: Peritoneal carcinomatosis (PC) presents a major challenge in the treatment of late-stage, solid tumors, with traditional therapies limited by poor drug penetration. We evaluated a novel hyperthermic pressurized intraperitoneal aerosol chemotherapy (HPIPAC) system using a human abdominal cavity model for its efficacy against AGS gastric cancer cells. Materials and Methods: A model simulating the human abdominal cavity and AGS gastric cancer cell line cultured dishes were used to assess the efficacy of the HPIPAC system. Cell viability was measured to evaluate the impact of HPIPAC under 6 different conditions: heat alone, PIPAC with paclitaxel (PTX), PTX alone, normal saline (NS) alone, heat with NS, and HPIPAC with PTX. Results: Results showed a significant reduction in cell viability with HPIPAC combined with PTX, indicating enhanced cytotoxic effects. Immediately after treatment, the average cell viability was 66.6%, which decreased to 49.2% after 48 hours and to a further 19.6% after 120 hours of incubation, demonstrating the sustained efficacy of the treatment. In contrast, control groups exhibited a recovery in cell viability; heat alone showed cell viability increasing from 90.8% to 94.4%, PIPAC with PTX from 82.7% to 89.7%, PTX only from 73.3% to 74.8%, NS only from 90.9% to 98.3%, and heat with NS from 74.4% to 84.7%. Conclusions: The HPIPAC system with PTX exhibits a promising approach in the treatment of PC in gastric cancer, significantly reducing cell viability. Despite certain limitations, this study highlights the system's potential to enhance treatment outcomes. Future efforts should focus on refining HPIPAC and validating its effectiveness in clinical settings.

키워드

과제정보

This study received funding from Seoul National University Bundang Hospital (SNUBH 14-2019-0004, 14-2020-0004).

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