한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제39권3호
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  • Pages.266-272
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  • 2024
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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어린이집 급식설비에서 분리된 식중독 미생물의 독소 유전자 및 항생제 내성

Toxin Genes and Antibiotic Resistance of Food Poisoning Bacteria Isolated from Food Service Equipment in Childcare Centers

  • 김은영 (국립순천대학교 식품공학과) ;
  • 김채영 (국립순천대학교 식품공학과) ;
  • 임지유 (국립순천대학교 식품공학과) ;
  • 김중범 (국립순천대학교 식품공학과)
  • Eun-yeong Kim (Department of Food Science and Technology, Sunchon National University) ;
  • Chae-Young Kim (Department of Food Science and Technology, Sunchon National University) ;
  • Ji-Yu Im (Department of Food Science and Technology, Sunchon National University) ;
  • Jung-Beom Kim (Department of Food Science and Technology, Sunchon National University)
  • 투고 : 2024.05.19
  • 심사 : 2024.06.12
  • 발행 : 2024.06.30
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초록

본 연구는 어린이집 급식설비 손잡이의 식중독 세균 오염도를 측정하고, 분리 균주의 독소 유전자와 항생제 내성을 분석하여 급식설비 손잡이에 의한 집단식중독을 예방을 위한 과학적 기반을 마련하고자 하였다. 실험 대상은 전라남도 일부 지역 어린이집 101곳의 냉장고, 냉동고, 자외선 살균기 손잡이, 총 303개를 대상으로 하였다. 어린이집 냉장고, 냉동고 손잡이에서 B. cereus 4 균주(1.3%)가 검출되었고 어린이집 냉장고 손잡이에서 S. aureus 2균주(0.7%)가 검출되었다. B. cereus와 S. aureus의 독소유전자를 분석한 결과 B. cereus 4개 균주 모두에서 nheA, nheB, nheC, entFM, cytK가 검출되었으나, S. aureus의 2개 균주의 경우 모두 sea, seb, sec, sed, see, seg, seh, sei, sej 독소유전자가 불검출되었다. B. cereus에서 설사를 유발하는 장독소가 검출되어 B. cereus에 의한 식중독 발생가능성이 상존하는 것으로 판단되었다. B. cereus와 S. aureus의 항생제 감수성을 실험한 결과 B. cereus 4 균주에서 AM, FEP 등 β-lactam계 항생제에 내성을 나타내었고 S. aureus 균주 모두 AM, P 항생제에 내성을 나타내었다. S. aureus 균주는 OX 항생제에 각각 중간 내성 1 균주와 감수성 1 균주를 나타내었으나 MRSA는 검출되지 않았다. 이러한 결과를 종합하여 볼 때 어린이집 급식설비 손잡이의 교차오염으로 인한 식중독 발생을 예방하기 위해 급식설비 손잡이에 대한 주기적인 살균 등 위생관리 방안을 강화해야할 것으로 판단되었다.

This study assessed the contamination level of food poisoning bacteria on handles of food service equipment in childcare centers to prevent food poisoning and analyzed toxin genes and antibiotic resistance of isolated strains. The isolates used in this study were collected from 101 childcare centers in Jeollanam-do. Four strains of Bacillus cereus and two strains of Staphylococcus aureus were isolated on the handles of food service equipment (refrigerators and freezers). The toxin genes of B. cereus were detected as nheA, nheB, nheC, entFM, and cytK. No toxin genes of S. aureus were detected. B. cereus showed resistance to β-lactam antibiotics, such as ampicillin and cefepime. S. aureus also showed resistance to antibiotics such as ampicillin and cefepime. Therefore, microbial safety and hygiene management, such as periodic sterilization of handles, should be strengthened to prevent food poisoning caused by cross-contamination of food service equipment handles in childcare centers.

키워드

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