Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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A Study on KASP Analysis Based on SNP to Rapidly Identify Caviar-Producing Sturgeon Species

캐비어를 생산하는 철갑상어의 신속 종판별을 위한 SNP 기반 KASP 분석에 관한 연구

  • Sun Hee Lee (Center for Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Bo Reum Park (Center for Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Hyung Il Kim (Deputy Director General for Food Standard Planning, Food Safety Policy Bureau, Ministry of Food and Drug Safety Ministry of Food and Drug Safety) ;
  • Sooyeul Cho (Medical Device Safety Management Division, Seoul Regional Office of Food and Drug Safety) ;
  • Kyung-Hun Son (Center for Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 이선희 (식품의약품안전처 식품의약품안전평가원 독성평가연구부 첨단분석과) ;
  • 박보름 (식품의약품안전처 식품의약품안전평가원 독성평가연구부 첨단분석과) ;
  • 김형일 (식품의약품안전처 식품안전정책국 식품기준기획관 첨가물기준과) ;
  • 조수열 (식품의약품안전처 서울지방식품의약품안전청 의료기기안전관리과) ;
  • 손경훈 (식품의약품안전처 식품의약품안전평가원 독성평가연구부 첨단분석과)
  • Received : 2024.05.08
  • Accepted : 2024.06.12
  • Published : 2024.06.30
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Abstract

Cavier is graded as Beluga, Osetra, and Sevruga based on the species of sturgeon (Acipenser sinensis). In this study, we developed an analytical method for determining the grade of black caviar using DNA barcodes and KASP markers. To identify the sturgeon species, ten black caviar samples were collected, and a reference DNA barcode library was developed using five genes (namely, 16S ribosomal RNA, cytochrome b, cytochrome c oxidase subunit I, cytochrome c oxidase subunit II, and NADH dehydrogenase subunit 5 genes). To develop the KASP markers, we selected 11 markers that could distinguish between the five grades of black caviar. As a result, specific markers for each of the targeted caviars were clustered into FAM-positive sections. DNA barcoding and the KASP assay revealed that one Beluga, six Osetra, and three Sevruga were identified among the ten caviar samples. Moreover, we found that the sturgeon species were mislabeled in two products. Here, we aimed to develop a KASP assay based on SNP that allows rapid and easy identification of caviar grade. These methods are expected to contribute to preventing the distribution of illegal products.

캐비어에 대한 수요가 증가하면서 캐비어 종류의 진위여부를 확인 할 수 있는 분석법 마련이 필요해졌다. 본 연구에서는 캐비어 종류을 나누는 철갑상어 종 특이 KASP 마커를 개발하였고 모니터링을 통해 국내 유통중인 불법 캐비어 제품을 확인하고자 하였다. 본 연구에서는 16S ribosomal RNA gene, cytochrome b gene, cytochrome coxidase subunit I gene, cytochrome c oxidase subunit II gene, NADH dehydrogenase subunit 5 gene에서 철갑상어종 특이적인 SNP를 선정하고 이를 표적하는 KASP 마커 11종을 개발하였다. 개발한 KASP 마커를 이용하여 한국의 온라인 마켓에서 유통중인 캐비어 10종을 모니터링 분석한 결과 2개의 제품에서 제품에 표기된 철갑상어 종과 다른 것으로 확인하였으며 두 제품 모두 실제보다 더 비싼 캐비어로 둔갑하여 판매한 것으로 확인되었다. 본 연구를 통해 제작한 KASP 분석방법으로 유통되는 캐비어 종류 분류가 가능하였고 모니터링을 통해 국내 유통되고 있는 불법 캐비어 제품도 확인하였다. 이에 따라 본 연구에서 개발한 SNP기반 KASP 분석법으로 불법 캐비어 제품 유통 근절에 기여 할 수 있을 것으로 기대한다.

Keywords

Acknowledgement

본 연구는 2021년와 2023년도 식품의약품안전처의 연구개발비(21201분석연307, 23211분석연241)로 수행되었으며, 이에 감사드립니다.

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