Journal of the Korea Institute of Military Science and Technology (한국군사과학기술학회지)

The Korea Institute of Military Science and Technology (한국군사과학기술학회)
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Rapid, Simultaneous Detection of Various Biological Toxin Genes Using Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification(RT-LAMP)

다중 역전사-루프매개등온증폭법(RT-LAMP)를 이용한 생물 독소 유전자 신속 진단법

  • Seungho Lee (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Chanho Chung (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Sehun Gu (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Jungeun Kim (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Hyeongseok Yun (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Daesang Lee (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Gyeunghaeng Hur (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development) ;
  • Donghyun Song (Advanced Defense Science & Technology Research Institute - Chem-Bio Technology Center, Agency for Defense Development)
  • 이승호 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 정찬호 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 구세훈 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 김정은 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 윤형석 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 이대상 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 허경행 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 송동현 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터)
  • Received : 2023.12.06
  • Accepted : 2024.05.13
  • Published : 2024.08.05
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Abstract

Rapid, early, accurate detection and identification of the various pathogenic agents associated with the development of biological weapons is critical in preventing loss of life and limiting the impact of these organisms when used against civilian or military targets. The aim of this study was to produce a system for the simple, rapid, accurate and simultaneous detection and identification of Ricin, Botulinum toxin B and Staphylococcal enterotoxin B as a proof of principle for developing field appropriate reverse transcription loop-mediated isothermal amplification systems for the accurate identification of potential biological threats. These systems were designed to facilitate the identification of potential threats even in remote or resource-limited locations.

Keywords

Acknowledgement

본 논문은 2024년 정부의 재원으로 국방과학연구소에서 수행한 연구 결과임(912A01201).

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