식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

마늘 유전자원의 Stemphylium vesicarium에 의한 잎마름병 저항성 평가

Evaluation of Garlic Germplasm for Resistance to Leaf Blight Caused by Stemphylium vesicarium

  • 이진주 (한국화학연구원 친환경신물질연구센터) ;
  • 한지원 (국립원예특작과학원 파속채소연구센터) ;
  • 김헌 (한국화학연구원 친환경신물질연구센터) ;
  • 김진철 (전남대학교 농생명화학과) ;
  • 최경자 (한국화학연구원 친환경신물질연구센터)
  • Jin Ju Lee (Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • JiWon Han (Allium Vegetable Research Center, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hun Kim (Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Jin-Cheol Kim (Department of Agricultural and Biological Chemistry, Chonnam National University) ;
  • Gyung Ja Choi (Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology)
  • 투고 : 2024.04.18
  • 심사 : 2024.05.16
  • 발행 : 2024.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Stemphylium vesicarium에 의해 발병하는 잎마름병은 마늘재배에 있어 전 세계적으로 가장 중요한 곰팡이 병 중 하나이며, 종자의 품질과 수확량을 감소시킨다. 병 저항성 품종의 재배는 화학 농약의 사용을 감소시키고, 작물 손실을 최소화하는 데 효율적인 방법이다. 본 연구는 잎마름병 저항성 마늘 유전자원을 발굴하기 위하여, 20점의 마늘 유전자원의 잎마름병에 대한 저항성을 조사하기 위하여 실험하였다. 온실에서 재배한 4엽기 유묘의 잎을, 접종 직전에 탈지하지 않은 비흡수성 재질의 솜으로 문지른 후에 멸균한 1/2 농도의 PDB 용액으로 수확한 S. vesicarium KACC 44530 균주의 포자현탁액(3.0×105 spores/ml)을 분무하여 접종하였다. 그리고 접종 3-7일 후에 잎에 발생한 잎마름병의 병반면적률(%)을 조사하였다. 이때 저항성 및 감수성 대조 품종으로 '남도'와 '대서' 마늘이 사용되었다. 실험한 유전자원 중에서, IT245512, IT245528 및 IT244068은 잎마름병에 가장 높은 저항성을 보였으며, 유전자원 IT257134와 IT253043은 잎마름병에 대하여 가장 높은 감수성을 나타내었다. 본 연구에서 선발한 저항성 유전자원은 잎마름병 저항성 육종 시스템에 유용한 자원으로 이용될 수 있을 것이다.

Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize crop losses. In this study, to find the resistant garlic resources against S. vesicarium, we evaluated the resistance degree of 20 garlic germplasms. To do this, garlic seedlings at four-leaf stage were rubbed with nonabsorbent cotton and then inoculated with spore suspension (3.0×105 spores/ml of potato dextrose broth) of S. vesicarium by spray method. Three to seven days after inoculation, the infected leaf area (%) of garlic seedling was measured. 'Daeseo' and 'Namdo' were included as susceptible and resistant control cultivars, respectively. After 3 to 7 days of incubation, the infected leaf area (%) of garlic seedling was measured. Our results showed that IT245512, IT245528, and IT244068 lines exhibited the highest resistance against S. vesicarium, whereas IT257134 and IT253043 lines were more susceptible than the susceptible cultivar 'Daeseo'. Based on the results, the resistant genetic resources selected in this study can be used a basic material for resistant garlic breeding system against leaf blight.

키워드

과제정보

This study was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project no. PJ01481604), the Rural Development Administration, Republic of Korea.

참고문헌

  1. Alberoni, G., Cavallini, D., Collina, M. and Brunelli, A. 2010. Characterisation of the first Stemphylium vesicarium isolates resistant to strobilurins in Italian pear orchards. Eur. J. Plant Pathol. 126: 453-457.
  2. Bae, R.-N., Choi, S.-Y. and Hong, Y.-P. 2008. The qualities of northern and southern ecotype garlic bulbs at different storage temperature. Korean J. Food Preserv. 15: 635-641. (In Korean)
  3. Barboza, K., Salinas, M. C., Acuna, C. V., Bannoud, F., Beretta, V., Garcia- Lampasona, S. et al. 2020. Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids. Sci. Hortic. 261: 108900.
  4. Basallote, M. J., Prados, A. M., Perez de Algaba, A. and Melero-Vara, J. M. 1993. First report in Spain of two leaf spots of garlic caused by Stemphylium vesicarium. Plant Dis. 77: 952.
  5. Basallote-Ureba, M. J., Prados-Ligero, A. M. and Melero-Vara, J. M. 1999. Aetiology of leaf spot of garlic and onion caused by Stemphylium vesicarium in Spain. Plant Pathol. 48: 139-145.
  6. Cho, H. S. and Yu, S. H. 1998. Stemphylium vesicarium on garlic and other Allium spp. in Korea. Korean J Plant Pathol. 14: 567-570.
  7. Corzo-Martinez, M., Corzo, N. and Villamiel, M. 2007. Biological properties of onions and garlic. Trends Food Sci. Technol. 18: 609-625.
  8. Egea, L. A., Merida-Garcia, R., Kilian, A., Hernandez, P. and Dorado, G. 2017. Assessment of genetic diversity and structure of large garlic (Allium sativum) germplasm bank, by diversity arrays technology "Genotyping-by-Sequencing" Platform (DArTseq). Front. Genet. 8: 98.
  9. Falloon, P. G., Falloon, L. M. and Grogan, R. G. 1987. Etiology and epidemiology of Stemphylium leaf spot and purple spot of asparagus in California. Phytopathology 77: 407-413.
  10. Figliuolo, G., Candido, V., Logozzo, G., Miccolis, V. and Spagnoletti Zeuli, P. L. 2001. Genetic evaluation of cultivated garlic germplasm (Allium sativum L. and A. ampeloprasum L.). Euphytica 121: 325-334.
  11. Foster, J. M. and Hausbeck, M. K. 2010. Resistance of pepper to Phytophthora crown, root, and fruit rot is affected by isolate virulence. Plant Dis. 94: 24-30.
  12. Grinstein, A., Frankel, H., Austerweil, M. and Kritzman, G. 1988. Airassisted placement spraying for Stemphylium control in seed onions. Crop Prot. 7: 356-360.
  13. Hassan, M., Yousuf, V., Shah, T. A., Bhat, N. A., Bhat, Z. A., Majeed, M. et al. 2020. Stemphylium blight of onion: a review. Agric. Rev. 41: 51-58.
  14. Hay, F., Stricker, S., Gossen, B. D., McDonald, M. R., Heck, D., Hoepting, C. et al. 2021. Stemphylium leaf blight: a re-emerging threat to onion production in eastern North America. Plant Dis. 105: 3780-3794.
  15. Hay, F. S., Sharma, S., Hoepting, C., Strickland, D., Luong, K. and Pethybridge, S. J. 2019. Emergence of Stemphylium leaf blight of onion in New York associated with fungicide resistance. Plant Dis. 103: 3083-3092.
  16. Jeger, M. J. and Viljanen-Rollinson, S. L. H. 2001. The use of the area under the disease-progress curve (AUDPC) to assess quantitative disease resistance in crop cultivars. Theor. Appl. Genet. 102: 32-40.
  17. Jo, E. J., Jang, K. S., Choi, Y. H., Ahn, K. G. and Choi, G. J. 2016. Resistance of cabbage plants to isolates of Plasmodiophora brassicae. Hortic. Sci. Technol. 34: 442-452. (In Korean)
  18. Jo, S.-J., Shim, S.-A., Jang, K. S., Choi, Y. H., Kim, J.-C. and Choi, G. J. 2014. Resistance of chili pepper cultivars to isolates of Phytophthora capsici. Hortic. Sci. Technol. 32: 66-76. (In Korean)
  19. Kim, J.-S. 2021. Phytochemical composition and physiological activity of the new garlic (Allium sativum) cv. 'Jangsaemi'. Korean J. Food Sci. Technol. 53: 701-705. (In Korean)
  20. Kim, J.-S., Ra, J. H. and Hyun, H.-N. 2015. Comparison of biochemical composition and antimicrobial activity of southern-type garlic grown in the eastern and western regions of Jeju. Hortic. Sci. Technol. 33: 763-771. (In Korean)
  21. Koike, S. T., O'Neill, N., Wolf, J., Van Berkum, P. and Daugovish, O. 2013. Stemphylium leaf spot of parsley in California caused by Stemphylium vesicarium. Plant Dis. 97: 315-322.
  22. Kumar, U., Naresh, P. and Kumar, S. 2020. Epidemiology and management of Stemphylium blight of garlic caused by Stemphylium vasicarium (Wallr.). J. Entomol. Zool. Stud. 8: 1006-1011.
  23. Lamprecht, S. C., Baxter, A. and Thompson, A. H. 1984. Stemphylium vesicarium on Medicago spp. in South Africa. Phytophylactica 16: 73-75.
  24. Lee, J. H., Lee, J. and Oh, D.-G. 2018. Resistance of pepper cultivars to Ralstonia solanacearum isolates from major cultivated areas of chili peppers in Korea. Hortic. Sci. Technol. 36: 569-576. (In Korean)
  25. Lee, J. J., Choi, Y. H., Kim, H., Kim, J.-C. and Choi, G. J. 2022a. Development of an in vivo bioassay method on leaf blight of onion caused by Stemphylium vesicarium. Korean J. Pestic. Sci. 26: 83-93. (In Korean)
  26. Lee, J. J., Kim, H., Kim, J.-C. and Choi, G. J. 2022b. A bioassay for testing garlic resistance to leaf blight caused by Stemphylium vesicarium. Hortic. Sci. Technol. 40: 324-337. (In Korean)
  27. Lee, J. S., Park, Y. U., Jeong, J. H., Kwon, Y. H., Chang, W. B. and Lee, H. D. 2021. Classification of garlic germplasms based on agronomic characteristics and multivariative analysis. Korean J. Plant Res. 34: 79-88. (In Korean)
  28. Lee, S. M., Lee, J. H., Jang, K. S., Choi, Y. H., Kim, H. and Choi, G. J. 2020. Resistance of commercial radish cultivars to isolates of Fusarium oxysporum f. sp. raphani. Hortic. Sci. Technol. 38: 97-106. (In Korean)
  29. Lee, S. M., Lee, J. J., Kim, H. and Choi, G. J. 2024. Differential resistance of radish cultivars against bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum. Plant Pathol. J. 40: 151-159.
  30. Llorente, I. and Montesinos, E. 2006. Brown spot of pear: an emerging disease of economic importance in Europe. Plant Dis. 90: 1368-1375.
  31. Mehta, Y. R. 1998. Severe outbreak of Stemphylium leaf blight, a new disease of cotton in Brazil. Plant Dis. 82: 333-336.
  32. Miller, M. E. and Schwartz, H. F. 2008. Stemphylium leaf blight and stalk rot. In: Compendium of Onion and Garlic Diseases and Pests, 2nd ed., eds. by H. F. Schwartz and S. K. Mohan, pp. 45-47. APS Press, St. Paul, MN, USA.
  33. Miller, M. E., Taber, R. A. and Amador, J. M. 1978. Stemphylium blight of onion in South Texas. Plant Dis. Rep. 62: 851-853.
  34. Mishra, B. and Singh, R. P. 2019. Reaction of onion varieties to Stemphylium blight (Stemphylium vesicarium). Int. J. Curr. Microbiol. App. Sci. 8: 1875-1880.
  35. Mishra, R. K., Jaiswal, R. K., Kumar, D., Saabale, P. R. and Singh, A. 2014. Management of major diseases and insect pests of onion and garlic: a comprehensive review. J. Plant Breed. Crop Sci. 6: 160-170.
  36. Nair, A., Khar, A., Hora, A. and Malik, C. P. 2013. Garlic: its importance and biotechnological improvement. Int. J. Life Sci. 2: 77-89.
  37. Panse, R., Jain, P. K., Gupta, A. and Sasode, D. S. 2013. Morphological variability and character association in diverse collection of garlic germplasm. Afr. J. Agric. Res. 8: 2861-2869.
  38. Papaioannou, C., Fassou, G., Petropoulos, S. A., Lamari, F. N., Bebeli, P. J. and Papasotiropoulos, V. 2023. Evaluation of the genetic diversity of Greek garlic (Allium sativum L.) accessions using DNA markers and association with phenotypic and chemical variation. Agriculture 13: 1408.
  39. Pattori, E., Rossi, V., Bugiani, R. and Giosue, S. 2006. Virulence of Stemphylium vesicarium isolates from pear and other host species. IOBC/wprs Bull. 29: 195-205.
  40. Prados-Ligero, A. M., Gonzalez-Andujar, J. L., Melero-Vara, J. M. and Basallote-Ureba, M. J. 1998. Development of Pleospora allii on garlic debris infected by Stemphylium vesicarium. Eur. J. Plant Pathol. 104: 861-870.
  41. Prados-Ligero, A. M., Melero-Vara, J. M., Corpas-Hervias, C. and Basallote-Ureba, M. J. 2003. Relationships between weather variables, airborne spore concentrations and severity of leaf blight of garlic caused by Stemphylium vesicarium in Spain. Eur. J. Plant Pathol. 109: 301-310.
  42. Ryu, Y.-H., Huh, C.-S., Kim, D.-G., Yeon, I.-K., Jo, W.-S. and Ryu, J.-A. 2015. Controlling effect of some environmentally friendly agents on garlic leaf blight in garlic. Korean J. Organic Agri. 23: 347-357. (In Korean)
  43. Sharma V, R., Malik, S., Kumar, M. and Sirohi, A. 2018. Assessment of genetic diversity in garlic (Allium sativum L.) genotypes based on ISSR markers. J. Pharmacogn. Phytochem. 7: 3119-3124.
  44. Shin, J.-H., Ju, J.-C., Kwen, O.-C., Yang, S.-M., Lee, S.-J. and Sung, N.-J. 2004. Physicochemical and physiological activities of garlic from different area. Korean J. Food Nutr. 17: 237-245. (In Korean)
  45. Shishkoff, N. and Lorbeer, J. W. 1989. Etiology of Stemphylium leaf blight of onion. Phytopathology 79: 301-304.
  46. Song, Y. S., Choi, I. H., Jang, Y. S., Ahn, Y. S., Cho, S. K. and Choi, W. Y. 2001. Relationship with major physiological characters and RAPD patterns of garlic (Allium sativum L.) germplasm. Korean J. Plant Res. 14: 139-147.
  47. Sultan, S. M. and Raina, S. K. 2020. Agro-morphological characterization of local garlic (Allium sativum L.) germplasm accessions collected from different regions of Jammu and Kashmir. J. Appl. Nat. Sci. 12: 124-127.
  48. Wang, H., Wu, Y., Liu, X., Du, Z., Qiu, Y., Song, J. et al. 2019. Resistance and clonal selection among Allium sativum L. germplasm resources to Delia antiqua M. and its correlation with allicin content. Pest Manag. Sci. 75: 2830-2839.
  49. Zheng, L., Lv, R., Huang, J., Jiang, D., Liu, X. and Hsiang, T. 2010. Integrated control of garlic leaf blight caused by Stemphylium solani in China. Can. J. Plant Pathol. 32: 135-145.