The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Anti-cancer Effects and Changes in Colonic Microflora of Polysaccharide Derived from Edible Mushroom Mycelium on AOM/DDS-induced Colon Cancer Model

AOM/DDS로 대장암 유도 후 식용버섯균사체 유래 다당류의 대장암 억제효과 및 대장 미생물균총의 변화

  • Seaung Sik Kong (Institute of Health Industry, Hoseo University) ;
  • Soon Ah Kang (Dept. of Convergence Technology, Graduate School of Venture, Hoseo University)
  • 공승식 (호서대학교 보건산업연구소 ) ;
  • 강순아 (호서대학교 벤처대학원 융합공학과)
  • Received : 2024.05.03
  • Accepted : 2024.06.05
  • Published : 2024.06.30
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Abstract

The objective of this study was to investigate the anticancer effects of EMPS (edible mushroom mycelium polysaccharide: Tremella fuciformis) in animal models with colorectal cancer induced by AOM/DSS. The experimental groups consisted of Nor (normal), NC (AOM/DSS), EMPS (EMPS 50, EMPS 100), and PC (Fluorouracil). The NC group had the highest number of colon tumors, whereas it was observed that tumor occurrence was significantly reduced in the EMPS consumption group. The expression of Bcl-2, an apoptosis inhibitor, was significantly lower in the EMPS 50 & 100 and PC groups. On the other hand, the mRNA gene expression of Bax, a factor that induces apoptosis, was significantly higher in the EMPS 50 & 100 and PC groups compared to the NC group. The mRNA expression levels of TNF-α and COX-2 significantly increased in the NC group, but showed a significant decrease in the EMPS and PC groups, indicating inhibition of the cancer-promoting response of cells. At the phylum level of the mice's intestinal microbial composition, the proportion of Bacteroidetes tended to decrease, while the proportion of Firmicutes tended to increase with EMPS administration. This suggests that changes in the gut microbiota caused by inflammation can be influenced by dietary intake.

Keywords

Acknowledgement

본 논문은 한국생명공학연구원 지원(KGM5482113)을 받아 수행된 연구로 이에 감사드립니다.

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