Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The cooperative regulatory effect of the miRNA-130 family on milk fat metabolism in dairy cows

  • Xiaofen Li (School of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College) ;
  • Yanni Wu (College of Animal Science and Technology, Yangzhou University) ;
  • Xiaozhi Yang (School of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College) ;
  • Rui Gao (College of Animal Science and Technology, Yangzhou University) ;
  • Qinyue Lu (Laboratory of Animal Developmental Biology, Department of Animal Science, Chungbuk National University) ;
  • Xiaoyang Lv (Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University) ;
  • Zhi Chen (College of Animal Science and Technology, Yangzhou University)
  • Received : 2023.11.15
  • Accepted : 2024.03.02
  • Published : 2024.07.01
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Abstract

Objective: There is a strong relationship between the content of beneficial fatty acids in milk and milk fat metabolic activity in the mammary gland. To improve milk quality, it is therefore necessary to study fatty acid metabolism in bovine mammary gland tissue. In adipose tissue, peroxisome proliferator-activated receptor gamma (PPARG), the core transcription factor, regulates the fatty acid metabolism gene network and determines fatty acid deposition. However, its regulatory effects on mammary gland fatty acid metabolism during lactation have rarely been reported. Methods: Transcriptome sequencing was performed during the prelactation period and the peak lactation period to examine mRNA expression. The significant upregulation of PPARG drew our attention and led us to conduct further research. Results: According to bioinformatics prediction, dual-luciferase reporter system detection, real-time quantitative reverse transcription polymerase chain reaction and Western blotting, miR-130a and miR-130b could directly target PPARG and inhibit its expression. Furthermore, triglyceride and oil red O staining proved that miR-130a and miR-130b inhibited milk fat metabolism in bovine mammary epithelial cells (BMECs), while PPARG promoted this metabolism. In addition, we also found that the coexpression of miR-130a and miR-130b significantly enhanced their ability to regulate milk fat metabolism. Conclusion: In conclusion, our findings indicated that miR-130a and miR-130b could target and repress PPARG and that they also have a functional superposition effect. miR-130a and miR-130b seem to synergistically regulate lipid catabolism via the control of PPARG in BMECs. In the long-term, these findings might be helpful in developing practical means to improve high-quality milk.

Keywords

Acknowledgement

This study was supported by Taizhou Science and Technology Support Plan (Agriculture) Project (TN202313) and the National Natural Science Foundation of China (Grant Nos. 32272825), the Open Project Program of the International Joint Research Laboratory of the Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement (IJRLD-KF202210) and, Independent Innovation in Jiangsu Province of China (CX (21) 3119); "Qing Lan Project" and the"High-end talent support program" of Yangzhou University, China.

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