Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Enhancement of cryopreserved rooster semen and fertility potential after oral administration of Thai ginger (Kaempferia parviflora) extract in Thai native chickens

  • Vibuntita Chankitisakul (Department of Animal Science, Faculty of Agricultural, Khon Kaen University) ;
  • Supakorn Authaida (Department of Animal Science, Faculty of Agricultural, Khon Kaen University) ;
  • Wuttigrai Boonkum (Department of Animal Science, Faculty of Agricultural, Khon Kaen University) ;
  • Sarunya Tuntiyasawasdikul (Center for Research and Development of Herbal Health Products, Faculty of Pharmaceutical Sciences, Khon Kaen University)
  • Received : 2024.01.03
  • Accepted : 2024.02.07
  • Published : 2024.07.01
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Abstract

Objective: Semen cryopreservation is an effective method of preserving genetic material, particularly in native chicken breeds facing a substantial decline. In this study, we evaluated the quality of frozen/thawed rooster semen treated with different concentrations of oral administrations of black ginger (Kaempferia parviflora: KP) extract and determined its fertility. Methods: Thirty-two Thai native roosters (Pradu Hang Dum, 42 weeks old) were used in this study. The treatments were classified into four groups according to the concentration of KP extract administered to the roosters: 0, 100, 150, and 200 mg/kg body weight. The quality of fresh semen was analyzed before cryopreservation. Post-thaw sperm quality and fertility potential were determined. Also, lipid peroxidation was determined. Results: The results showed that sperm concentration and movement increased in roosters treated with 200 mg/kg of KP extract (p<0.05). The malondialdehyde (MDA) in the roosters receiving 200 mg/kg KP extract was lower than that in the other but had an insignificant difference within the KP treatment groups (p>0.05). The highest MDA levels were observed in the control group (p<0.05). The percentage of motile sperm (total motility and progressive motility) after semen thawing was higher in roosters that received 150 and 200 mg/kg KP extract than in those that received 100 mg/kg KP extract and the control (p<0.05). MDA levels decreased significantly in roosters that received 150 and 200 mg/kg KP extract than in those that received 100 mg/kg KP extract and the control (p<0.05). Fertility and hatchability were greater in the KP150 and KP200 groups than in the KP100 and control groups (p<0.05). Conclusion: The optimal amount of KP extract influencing initial sperm quality was determined to be 200 mg/kg. However, 150 mg/kg was the optimal low dosage of KP extract administration that maintained sperm quality and fertility following semen cryopreservation.

Keywords

Acknowledgement

Authaida, S was granted by the Graduate School Khon Kaen University through the Research Fund for Supporting Lecturers to Admit High Potential Students to Study and Research in His Expert Program Year 2021, grant no. 641JT103. The fundamental fund of Khon Kaen University and Thailand Science Research and Innovation funded this research.

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