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Isolation and Characterization of Plant Growth Promoting Bacteria Pseudomonas sp. SH-26 from Peat Soil

이탄 토양으로부터 식물생육촉진세균 Pseudomonas sp. SH-26의 분리 및 특성

  • Ho-Young Shin (Department of Microbial Biotechnology, College of Mokwon University) ;
  • Da-Son Kim (Department of Microbial Biotechnology, College of Mokwon University) ;
  • Chang-Ho Lee (DAEJI Development Co., Ltd.) ;
  • Dong-Soek Lee (DAEJI Development Co., Ltd.) ;
  • Song-Ih Han (Department of Microbial Biotechnology, College of Mokwon University)
  • 신호용 (목원대학교 미생물생명공학과) ;
  • 김다솜 (목원대학교 미생물생명공학과) ;
  • 이창호 ((주)대지개발) ;
  • 이동석 ((주)대지개발) ;
  • 한송이 (목원대학교 미생물생명공학과)
  • Received : 2024.02.12
  • Accepted : 2024.04.15
  • Published : 2024.04.30

Abstract

We conducted to investigate both plant growth-promoting and plant disease-controlling activities of bacterial strains isolated from soil. Among the 48 isolated strains, SH-23, SH-26, SH-29, and SH-33 were identified as excellent strains for the production of β-glucosidase, cellulase, amylase, and protease. These 4 strains exhibited antifungal activity against plant pathogenic fungi (Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Colletotrichum acutatum). Strain SH-26, which exhibited excellent organic matter decomposition and antifungal activity against plant pathogenic fungi, was selected as the final superior strain. Upon determining the 16S rRNA gene sequence of the selected SH-26 strain, it exhibited 100% similarity with Pseudomonas knackmussii HG322950 B13T, Pseudomonas citronellolis BCZY01000096 NBRC 103043T, and Pseudomonas delhiensis jgi.1118306 RLD-1T. Furthermore, it was confirmed that the Pseudomonas sp. SH-26 exhibited siderophore production, nitrogen fixation ability, and the production of Indole-3-acetic acid.

이탄 토양으로부터 분리된 세균의 유기물 분해능, 항균 활성 및 식물생육촉진능을 확인하고 다기능성 유용 미생물을 확보하고자 하였다. 분리된 48 균주를 대상으로 β-glucosidase, cellulase, amylase, protease 생성능을 확인한 결과, SH-23, SH-26, SH-29 그리고 SH-33 균주가 우수 균주로 선발되었다. 선발된 유기물 분해 우수 세균 4 균주는 식물병원성곰팡이(Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Colletotrichum acutatum)에 대한 항진균 활성을 나타내었다. 유기물 분해능이 우수하고 식물병원성 곰팡이에 대해 항진균 활성을 나타낸 SH-26 균주를 최종 우수균주로 선발 하였다. 선발된 SH-26 균주의 16S rRNA 유전자 염기 서열을 결정한 결과, Pseudomonas knackmussii HG322950 B13T, Pseudomonas citronellolis BCZY01000096 NBRC 103043T, 그리고 Pseudomonas delhiensis jgi.1118306 RLD-1T와 100%의 상동성을 나타내었다. Pseudomonas sp. SH-26 균주는 siderophore 생성능, 질소고정능과 Indole-3-acetic acid 생성능이 있는 것으로 확인되었다.

Keywords

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