International Journal of Industrial Entomology and Biomaterials

  • 제48권2호
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  • Pages.67-77
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  • 2024
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Effects of exogenous enzymes from invertebrate gut-associated bacteria on volatile organic compound emissions and microbiota in an in vitro pig intestine continuous fermentation model

  • Jong-Hoon Kim (Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ho-Yong Park (Insect Biotech Co. Ltd.) ;
  • Kwang-Hee Son (Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2023.11.22
  • 심사 : 2024.03.31
  • 발행 : 2024.06.30
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초록

This study aims to assess the efficacies of exogenous enzymes, derived from invertebrate gut-associated microbes, as feed additives, in reducing volatile organic compound (VOC) emissions using an in vitro pig intestine continuous fermentation system. An in vitro continuous fermentation model was used to simulate a comparable bionic digestion system by co-reacting feed, enzymatic additives (arazyme, mannanase, and xylanase, derived from the gut bacteria of Nephila clavata, Eisenia fetida, and Moechotypa diphysis, respectively), and gastrointestinal microbes, followed by an analysis of their correlations. A significant correlation was observed between exogenous enzyme supplementation and reduced VOC emissions in the fecal phase of continuous fermentation (p < 0.05). The concentration of VOCs decreased by 3.75 and 2.75 ppm in the treatment group following arazyme and multi-enzyme supplementation, respectively, compared to that in the control group (7.83 ppm). In addition, supplementation with arazyme and multiple enzymes significantly affected the microbial composition of each fermentation phase (p < 0.05). In particular, Lactiplantibacillus pentosus and Pediococcus pentosaceus, which changed in abundance according to arazyme or multi-enzyme supplementation, exhibited a positive relationship with VOC emissions. These results suggest that exogenous enzymes derived from invertebrate gut-associated bacteria can be efficiently applied as feed additives, leading to a reduction in VOC emissions.

키워드

과제정보

This work was carried out with the support of the Creative Allied Project (CAP-18-06-KRIBB) of National Research Council of Science & Technology (NST) grant by the Korea government (MSIT) and the KRIBB Research Initiative Program (KGM5492322) of the Ministry of Science and ICT, Republic of Korea.

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