Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Efficient Extracellular Secretion of the Antimicrobial Peptide Magainin 2 in the Chlorella-based System

클로렐라 시스템에서 항균펩타이드 Magainin 2의 효율적인 세포외 분비

  • 정유정 ((주)바이오이즈 중앙연구소) ;
  • 황재윤 ((주)바이오이즈 중앙연구소) ;
  • 김성천 ((주)바이오이즈)
  • Received : 2024.03.19
  • Accepted : 2024.04.09
  • Published : 2024.06.30
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Abstract

Various antimicrobial peptides (AMPs) from microalgae have shown antibacterial, antiviral, antifungal, anticancer, and antioxidant effects, and play crucial roles in medical applications, aquaculture-related disease management, and the food industry. Magainin 2 (MAG2), an AMP, exhibits high antibacterial and antitumor activity, necessitating an efficient recombinant expression system for low-cost, large-scale production. To enhance MAG2 secretion efficiency in Chlorella, we constructed the SS:MAG2:His vector using the known Chlamydomonas reinhardtii CA1 signal sequence (SS) and obtained a stable transformant via an Agrobacterium-mediated transformation method and RT-qPCR. ELISA results revealed that the MAG2 content secreted into the medium by the SS:MAG2:His transformants increased proportionally with mRNA expression. These findings offer a strategy for high MAG2 secretion in the Chlorella vulgaris platform, potentially minimizing downstream processing costs.

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References

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