Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Enhanced Biomass Productivity of Freshwater microalga, Parachlorella kessleri for Fixation of Atmospheric CO2 Using Optimal Culture Conditions

최적 배양 조건을 이용한 CO2 제거 목적의 담수 미세조류 Parachlorella kessleri의 바이오매스 생산성 향상

  • 김지훈 ((주)휴에버그린팜) ;
  • 홍선우 (인하대학교 생명공학과) ;
  • 김진우 (인하대학교 생명공학과) ;
  • 손병락 (대구경북과학기술원 에너지융합연구부) ;
  • 김미경 ((주)에코파이코텍) ;
  • 김용환 ((재)해양심층수산업 고성진흥원) ;
  • 설진현 ((주)워터코리아) ;
  • 전수환 ((주)휴에버그린팜)
  • Received : 2024.01.20
  • Accepted : 2024.03.15
  • Published : 2024.06.30
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Abstract

This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO2. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO2 fixation by increasing the availability of CO2(HCO3-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m2/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO2 availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO2 fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

Keywords

Acknowledgement

본 연구는 경상북도와 울진군의 '기후변화대응, 한국형 인공해초나무 도입 및 실증사업'의 지원과 과학기술정보통신부에서 지원하는 DGIST기관고유사업에 의해 수행되었습니다(24-ET-01).

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