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The Korean Society of Phycology (한국조류학회(藻類))
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Discovery of novel Nodosilinea species (Cyanobacteria, Nodosilineales) isolated from terrestrial habitat in Ryukyus campus, Okinawa, Japan

  • Handung Nuryadi (Tropical Biosphere Research Center, University of the Ryukyus) ;
  • Shimpei Sumimoto (Faculty of Chemistry and Biochemistry, Kanagawa University) ;
  • Shoichiro Suda (Department of Chemistry, Biology and Marine Science,Faculty of Science, University of the Ryukyus)
  • Received : 2023.12.05
  • Accepted : 2024.06.05
  • Published : 2024.06.19
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Abstract

Terrestrial cyanobacteria are extremely diverse. In urban areas, they can be found as black stains on the surface of building walls, stone monuments, or man-made structures. Many of the terrestrial cyanobacteria are still understudied. To expand knowledge of terrestrial cyanobacterial diversity, a polyphasic characterization was performed to identify 12 strains isolated from campus of University of the Ryukyus, Okinawa, Japan. Multigene phylogenetic analyses based on 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region showed that the isolated strains formed two independent subclades within Nodosilinea, and were distantly related to all described Nodosilinea species. The 16S-23S rRNA ITS secondary structures showed variations for D1-D1' and Box B domain, while V3 domain was almost identical among entire species of Nodosilinea, including the studied strains. In addition, a unique morphological character, i.e. forming nodule or spiral shape, was also observed in certain studied strains. According to polyphasic characterization, Nodosilinea coculeatus sp. nov. and Nodosilinea terrestrialis sp. nov., were proposed as two new species of terrestrial cyanobacteria from Okinawa.

Keywords

Acknowledgement

Part of this work was supported by JSPS KAKENHI Grant (#19K06090).

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