Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Combinational effects of sesquiterpene lactones isolated from bay laurel (Laurus nobilis) leaves with antibiotics against fish pathogenic bacteria

어류 병원성 세균에 대한 월계수(Laurus nobilis) 잎 유래 sesquiterpene lactone과 수산용 항생제의 병용효과

  • Jae-Woong Lim (Pathology Research Division, National Institute of Fisheries Science) ;
  • Ji-Seok Choi (Department of Aqualife Medicine, Chonnam National University) ;
  • Ayman Turk (College of Pharmacy, Chungbuk National University) ;
  • Mi Kyeong Lee (College of Pharmacy, Chungbuk National University) ;
  • Do-Hyung Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • So Young Kang (Department of Aqualife Medicine, Chonnam National University)
  • Received : 2024.05.28
  • Accepted : 2024.06.11
  • Published : 2024.06.30
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Abstract

This study is a report on combinational effects between four sesquiterpene lactones (SLs) from bay laurel (Laurus nobilis) leaves, and oxytetracycline (OTC) or amoxicillin (AMX) against four fish pathogenic bacteria such as Vibrio anguillarum, V. harveyi, Edwardsiella tarda, and Streptococcus iniae. Individually, four SLs exerted little antibacterial activity against fish pathogenic bacteria. However, when combined with OTC or AMX, they showed synergistic interaction against pathogenic bacteria. Especailly, zaluzanin C (1) reduced the MIC of OTC (or AMX) eight-fold. Our results showed that combinations of SLs with antibiotics (ABTs) are more effective than ABTs alone to control pathogenic bacteria. The highest synergistic effect was observed when zaluzanin C (1) was combined with OTC or AMX against V. harvey or S. iniae, displaying significant reductions of MICs up to 8-fold (0.125 to 0.015 ㎍/mL and 0.0078 to 0.0009 ㎍/mL). In addition, zaluzanin C (1) improved the antibiotic potency of OTC against OTC resistant V. harveyi (250 ㎍/mL to 62.5 ㎍/mL). Synergism between ABTs and phytochemical such as SLs could be a therapeutically helpful concept to improve the efficacy of ABTs and prevent antibiotic resistance. These results suggest that SLs can be used as an alternative to reduce antibiotic resistance in aquaculture.

Keywords

Acknowledgement

이 성과는 정부의 재원으로 한국연구재단(NRF-2019R1F1A1059303) 및 국립수산과학원 수산생물 질병 특성 연구(R2024054)의 지원을 받아 수행된 연구임.

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